Universal nucleic acid extraction kit and nucleic acid extraction method

A nucleic acid extraction reagent, a general-purpose technology, applied in biochemical equipment and methods, DNA preparation, and microbial determination/inspection, etc. Extraction yield and purity, ensuring integrity, reducing the effect of impurities

Active Publication Date: 2022-05-13
河北三狮生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems that the nucleic acid extraction kit in the prior art has a narrow scope of application, is not suitable for automatic operation, and the extraction effect is unstable, the present invention provides a general-purpose nucleic acid extraction kit and a nucleic acid extraction method

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  • Universal nucleic acid extraction kit and nucleic acid extraction method
  • Universal nucleic acid extraction kit and nucleic acid extraction method

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Embodiment 1

[0035] An embodiment of the present invention provides a universal nucleic acid extraction kit, the reagents of the nucleic acid extraction kit include a lysate, a first washing solution, a second washing solution and a nucleic acid eluent;

[0036] The lysate includes components of the following working concentrations: guanidine isothiocyanate 2.6mol / L, trishydroxymethylaminomethane 55mmol / L, isopropanol 30vol%, 200nm iron ferric oxide hydroxyl magnetic beads 5.0vol% , Triton X-100 10vol% and NP-40 3vol%; the pH of the lysate is 6.5-8;

[0037] The first eluent includes components of the following working concentrations: 2.25 mol / L of guanidine isothiocyanate, 30 mmol / L of trishydroxymethylaminomethane and 56 vol% of ethanol;

[0038] The second washing solution is 75% ethanol aqueous solution in volume percentage;

[0039] Nucleic acid eluent is DEPC water.

[0040] Utilize above-mentioned general type nucleic acid extraction kit to extract the method for porcine whole blo...

Embodiment 2

[0051] An embodiment of the present invention provides a universal nucleic acid extraction kit, the reagents of the nucleic acid extraction kit include a lysate, a first washing solution, a second washing solution and a nucleic acid eluent;

[0052] The lysate includes components of the following working concentrations: guanidine isothiocyanate 3.5mol / L, trishydroxymethylaminomethane 15mmol / L, isopropanol 40vol%, 200nm iron ferric oxide hydroxyl magnetic beads 6.5vol% , Triton X-100 8vol% and NP-40 5vol%; the pH of the lysate is 6.5-8;

[0053] The first eluent includes components of the following working concentrations: 3.63 mol / L of guanidine isothiocyanate, 100 mmol / L of trishydroxymethylaminomethane and 60 vol% of ethanol;

[0054] The second washing solution is 70% ethanol aqueous solution in volume percent;

[0055] The nucleic acid eluent is DEPC water and 100mmoL / L tris.

[0056] The method for extracting pig whole blood nucleic acid using the above-mentioned general...

Embodiment 3

[0065] An embodiment of the present invention provides a universal nucleic acid extraction kit, the reagents of the nucleic acid extraction kit include a lysate, a first washing solution, a second washing solution and a nucleic acid eluent;

[0066] The lysate includes the following components at working concentrations: 4.8 mol / L of guanidine isothiocyanate, 80 mmol / L of trishydroxymethylaminomethane, 27 vol% of isopropanol, and 9.8 vol% of ferric ferric oxide hydroxyl magnetic beads of 200 nm , Triton X-100 15vol% and NP-40 4vol%; the pH of the lysate is 6.5-8;

[0067] The first eluent includes components of the following working concentrations: 3.86mol / L of guanidine isothiocyanate, 10mmol / L of trishydroxymethylaminomethane and 55vol% of ethanol;

[0068] The second washing solution is 80% ethanol aqueous solution in volume percentage;

[0069] The nucleic acid eluent is DEPC water and 50mmoL / L tris.

[0070] The method for extracting pig whole blood nucleic acid using th...

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Abstract

The invention relates to the technical field of molecular biological detection, and particularly discloses a universal nucleic acid extraction kit and a nucleic acid extraction method. A lysis solution of the nucleic acid extraction kit comprises the following components with working concentrations: 2 mol/L to 5 mol/L of guanidine isothiocyanate, 15 mmol/L to 80 mmol/L of trihydroxymethyl aminomethane, 27 vol% to 40 vol% of an alcohol compound, 5 vol% to 10 vol% of ferroferric oxide hydroxyl magnetic beads with the particle size of 200 nm to 450 nm, 8 vol% to 15 vol% of Triton X-100 and 3 vol% to 5 vol% of NP-40. By adopting the nucleic acid extraction kit and the nucleic acid extraction method provided by the invention, rapid extraction of nucleic acids of various biological samples can be realized, the universality is relatively high, and the extracted nucleic acid fragments are complete, high in purity, high in yield and better in PCR (Polymerase Chain Reaction) detection effect, so that the nucleic acid extraction kit and the nucleic acid extraction method have relatively high popularization and application values.

Description

technical field [0001] The invention relates to the technical field of molecular biology detection, in particular to a general nucleic acid extraction kit and a nucleic acid extraction method. Background technique [0002] In recent years, the application of PCR detection technology has become more and more common, and is widely used in many fields such as medicine, food, animal husbandry, and aquatic products. Nucleic acid extraction is the basis of various molecular biology detection methods, and obtaining effective and reliable nucleic acid is the key to PCR detection technology. [0003] With the increase of detection technology and sample types, there are many kinds of nucleic acid detection kits on the market, such as bacterial extraction kits, tissue extraction kits, whole blood extraction kits, etc., and the extraction methods of each kit are also different. same. At present, most of the commercialized magnetic bead nucleic acid extraction kits need to use protease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/6806
CPCC12N15/1013C12Q1/6806C12Q2523/308C12Q2527/125
Inventor 柳梦思石磊王菁刘桐山时国强董振国
Owner 河北三狮生物科技有限公司
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