Primer group and kit for detecting human red blood cell Rh blood group genotyping and application

Abstract

The invention discloses a primer group and a kit for detecting blood type gene typing of human red blood cells Rh and application of the primer group. A sequence table of the primer group is as shown in SEQ ID No.1 to 9, SEQ ID No.10 to 12 or / and SEQ ID No.13 to 15, SEQ ID No.16 to 18 or / and SEQ ID No.19 to 21, SEQ ID No.22 to 24 or / and SEQ ID No.25 to 27, SEQ ID No.28 to 30, SEQ ID No.31 to 33 or / and SEQ ID No.34 to 36 and SEQ ID No.37 to 72. The primers are subjected to optimization design and have similar annealing temperatures, simultaneous amplification of all the primers under the same PCR amplification condition can be achieved, the volume of each PCR reaction system is only 1.15 microliters, only 5-10 ng of a DNA template is needed, and the detection efficiency and the reaction sensitivity are greatly improved; the primer group has the advantages of high speed, low cost, high sensitivity, easiness in realizing multi-site joint detection and the like when being applied to detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer set, a kit and an application for detecting human erythrocyte Rh blood type genotyping. Background technique [0002] In 1939, Levine and Stetson reported a case of hemolytic reaction: a pregnant woman had a stillbirth, and her husband’s blood was transfused postpartum. The ABO blood type was the same, and acute hemolytic reaction occurred after the blood transfusion, and antibodies against her husband’s red blood cells were isolated from her blood. This is The first Rh antibody discovered in humans. In 1940, Landsteiner and Wiener published the typing results of human erythrocytes by animal antisera, which is considered to be the first report on the Rh blood group system. Up to now, there are 43 red blood cell blood group systems approved by the International Society of Blood Transfusion (ISBT). The Rh blood group system is the most complex blood group system, and its imp...

AI Technical Summary

Problems solved by technology

The PCR-SSP method requires multi-tube amplification and multi-band electrophoresis, and there is also the risk of mistyping caused by confusing interpretation

The biggest defect of the PCR-SBT typing method is that the operation process is very cumbersome, the workload is heavy, and it is difficult to achieve high-throughput operation

The amplification of different regions of the Rh gene has different amplification conditions, which not only requires a large sample size, but also increases the consumption of reagent consumables, and requires more equipment resources.

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