Treatment method, detection method and application of beta-receptor stimulant residues in animal tissue

A receptor agonist and animal tissue technology, applied in the field of β-receptor agonist residues, can solve the problems of long solid phase extraction time, large detection deviation, and low work efficiency

Pending Publication Date: 2022-05-31
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, at present, the detection of β-receptor agonists in animal tissues, urine, and blood mainly adopts the method of enzymatic hydrolysis combined with solid-phase extraction (SPE for short), but the enzymatic hydrolysis time is often more than 16 hours, and the solid-phase The extraction time is also longer, especially when processing a large number of samples for the national "Clenbuterol" monitoring, the work efficiency is low, and the detection deviation caused by pure manual operation is also large

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  • Treatment method, detection method and application of beta-receptor stimulant residues in animal tissue
  • Treatment method, detection method and application of beta-receptor stimulant residues in animal tissue
  • Treatment method, detection method and application of beta-receptor stimulant residues in animal tissue

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Embodiment 1

[0086] The embodiment of the present application provides a treatment method and detection method for β-receptor agonist residues in animal tissues, including:

[0087] 1. Instruments and reagents

[0088] Waters Acquity XEVO TQS ultra-high performance liquid chromatography-tandem mass spectrometer, equipped with electrospray ion source (ESI), MassLynx 4.1 workstation (Waters, USA); HD 2200 ultrasonic generator (Bandelin, Germany), equipped with a diameter of 3mm titanium probe needle; RayKol Fotector Plus automatic solid-phase extraction instrument (Sage Scientific Instruments (Xiamen) Co., Ltd.); 3K15 high-speed refrigerated centrifuge (Germany Sigma Company); N-EVAP 112 nitrogen blowing instrument (American Organomation Company); Milli-Q ultrasonic Pure water instrument (Milli-Q Company, USA); BS 210S analytical balance (Sartorius Company, Germany); MS2 Minishaker vortex oscillator (IKA Company, Germany).

[0089] The 22 beta-agonists are clenbuterol, ractopamine, salbutam...

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Abstract

The invention provides a treatment method, a detection method and application of beta-receptor stimulant residues in animal tissues, and belongs to the technical field of food detection. The method for treating the beta-receptor stimulant residues in the animal tissues comprises the following steps: inserting an ultrasonic probe into a first mixed solution containing a sample, an internal standard substance, a buffer solution and beta-glucuronidase / aryl sulfatase, and performing ultrasonic probe-assisted enzymolysis for 1-5 minutes under the condition of output power of 700-1100W. According to the method for treating the beta-receptor stimulant residues in the animal tissues, the extraction efficiency of traditional 16-hour enzymolysis can be achieved within 5 minutes through ultrasonic probe assisted enzymolysis, the treatment time of the beta-receptor stimulant residues in the animal tissues is greatly shortened, and the method has the advantages of being more efficient, more accurate and more convenient.

Description

technical field [0001] This application relates to the technical field of food detection, in particular, to a treatment method, detection method and application of β-receptor agonist residues in animal tissues. Background technique [0002] Beta-receptor agonists, commonly known as "clenbuterol", belong to phenylethanolamine drugs. They are mainly used in clinical medicine to dilate bronchi and increase lung ventilation, and can treat respiratory diseases such as bronchial asthma. Because of their functions of accelerating fat decomposition, promoting protein synthesis and improving feed conversion rate, β-receptor agonists are illegally used in large quantities in animal farming to increase lean meat percentage. In addition, since β-receptor agonists (especially albuterol) have a significant dilating effect on animal capillaries, the amount of water the animals drink in a short period of time after feeding is greatly increased, achieving an effect similar to "water-injected...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02G01N30/72
CPCG01N30/06G01N30/02G01N30/72G01N2030/062G01N2030/045G01N2030/027
Inventor肖志明樊霞王石索德成李阳
OwnerINST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS