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Porous biological printing ink and preparation method thereof as well as body surface tissue and preparation method thereof

A bio-ink, tissue technology, applied in prosthesis, medical science, additive processing, etc., can solve the problem of hindering the exchange of nutrients, the mechanical stability of hydrogel is not enough to maintain accurate three-dimensional shape, and it is difficult to accurately simulate the cartilage microenvironment. and other issues to achieve the effect of promoting behavioral activities

Pending Publication Date: 2022-07-01
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the commonly used hydrogels currently used to construct auricle equivalents have the following defects: (1) the single component of traditional hydrogels is difficult to accurately simulate the unique microenvironment of cartilage; (2) the dense texture of cured hydrogels hinders exchange of nutrients and affect the formation of internal cartilage tissue; (3) the mechanical stability of the hydrogel is not enough to maintain a precise three-dimensional shape

Method used

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  • Porous biological printing ink and preparation method thereof as well as body surface tissue and preparation method thereof
  • Porous biological printing ink and preparation method thereof as well as body surface tissue and preparation method thereof
  • Porous biological printing ink and preparation method thereof as well as body surface tissue and preparation method thereof

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Embodiment 1

[0055] like Figure 1 to Figure 14 As shown, this embodiment provides a method for preparing a biomimetic porous bioink, which includes the following specific steps:

[0056] S1: pretreating cartilage tissue to obtain a tissue solution with a mass percentage of cartilage tissue of 5-10%;

[0057] S2: adding an appropriate amount of adjuvant to the above tissue solution and mixing to obtain a hydrogel solution with a stabilizer mass percentage of 5-10%;

[0058] S3: adding an appropriate amount of pore-forming agent to the above hydrogel solution and mixing to obtain a bioink with a mass percentage of 0.1-5% of the pore-forming agent.

[0059] The preparation process of this embodiment is simple, and the bioink provided by it has the following advantages: good biocompatibility, low immunogenicity, and mild in vivo inflammatory response; biomimetic simulation of cartilage-specific microenvironment; fast photocuring performance and The rheological properties are conducive to bi...

Embodiment 2

[0062] On the basis of Embodiment 1, in this embodiment, the S1 includes the following specific steps:

[0063] S11: After the cartilage tissue is cooled by liquid nitrogen, it is pulverized into cartilage powder by a pulverizer, and then decellularized and enzymatically digested to make cartilage acellular matrix;

[0064] S12: Weigh an appropriate amount of cartilage acellular matrix and dissolve it in an appropriate amount of deionized water to prepare an aqueous solution of cartilage acellular matrix with a mass percentage of 0.1-10%, and then add formazan at a rate of 0.1-1 mL / min under ice bath conditions methacrylic anhydride and mixing to obtain a mixed solution with a mass percentage of methacrylic anhydride of 0.1-1%;

[0065] Add sodium hydroxide with a concentration of 1 to 10 mol / L to maintain the pH value of the above mixed solution between 8 to 10, and continue to stir and react for 8 to 12 hours at 4°C in the dark;

[0066] After the reaction, neutralize to pH...

Embodiment 3

[0076] On the basis of any one of Embodiments 1 to 2, in this embodiment, the cartilage tissue in S1 is one or more of ear cartilage, articular cartilage, costal cartilage, scapular cartilage and meniscus. The invention is convenient to obtain and is beneficial to the preparation of biomimetic reconstructed body surface tissue.

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Abstract

The invention relates to bionic porous bio-ink and a preparation method thereof as well as body surface tissue and a preparation method thereof, and the preparation method of the bionic porous bio-ink comprises the following specific steps: pretreating cartilage tissue to obtain a tissue solution with the cartilage tissue mass percent of 5-10%; adding a proper amount of an auxiliary agent into the tissue solution, and uniformly mixing to obtain a hydrogel solution with the mass percent of the auxiliary agent being 5-10%; and adding a proper amount of a pore-forming agent into the hydrogel solution, and uniformly mixing to obtain the bio-ink with the mass percent of the pore-forming agent being 0.1-5%. The biological ink is good in biological form, good in biomechanical strength, beneficial to maintenance of a three-dimensional form, moderate in degradation rate and matched with a cartilage regeneration rate, and meanwhile, a body surface tissue substitute with an accurate form and suitable biological properties is prepared by combining a three-dimensional biological printing technology. A mature tissue is constructed in vivo / in vitro by applying the substitute, so that clinical transformation breakthrough is finally realized, and the clinical application requirement of body surface tissue reconstruction is met.

Description

technical field [0001] The invention relates to the technical field of three-dimensional bioprinting and tissue and organ repair and reconstruction, in particular to a porous bioprinting ink and a preparation method thereof, as well as a body surface tissue and a preparation method thereof. Background technique [0002] Defects or deletions of the auricle due to congenital microtia, trauma and other reasons are common clinically. The auricle is one of the main features of the human face, and its defect or absence can seriously affect the patient's aesthetics and mental health. At present, the most effective treatment methods are auricular reconstruction based on autologous costal cartilage carving and artificial prosthesis, but the former will cause severe surgical trauma to the donor site and the shape of the auricle is difficult to precisely control, while the latter is prone to foreign body rejection and No biological function. Therefore, there is an urgent need for an ...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/50A61L27/36B33Y70/10B33Y80/00
CPCA61L27/56A61L27/50A61L27/3654A61L27/3612A61L27/3633A61L27/3687A61L27/3691B33Y70/10B33Y80/00A61L2430/06A61L2430/40
Inventor 蒋海越刘霞贾立涛周广东华宇杰
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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