Application of hexanoic acid and hexyl hexanoate in repelling solenopsis invicta

A technology of hexyl caproate and red fire ants, which is applied in the fields of application, animal repellent, and pest repellent, can solve the problems of few types, high toxicity of humans and animals, etc., achieve good repellent effect, important market application and The effect of promotional potential

Active Publication Date: 2022-07-05
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of the rapid spread of red imported fire ants and the increasingly rampant damage, but in the existing quarantine monitoring technology of red imported imported fire ants, there are not many types of repellent agents for red imported fire ants, and they are highly toxic to humans and animals. Natural compound medicament with strong repelling effect on ants and its application

Method used

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  • Application of hexanoic acid and hexyl hexanoate in repelling solenopsis invicta
  • Application of hexanoic acid and hexyl hexanoate in repelling solenopsis invicta
  • Application of hexanoic acid and hexyl hexanoate in repelling solenopsis invicta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: GC-MS separation and identification of thymus gland compounds

[0021] S1. Extraction of glandular compounds by solution leaching

[0022] Use sharp tweezers to stimulate the adult P. figure 1 ) secreted yellow chemical defense substances, adsorbed the yellow secretions with capillary tubes, and dissolved the liquid secretions adsorbed by capillary tubes in a sample bottle containing 1 mL of chromatographically pure hexane. Then a small amount of anhydrous Na was added 2 SO 4 Shake for 2 min to remove excess water in the sample, filter the solution with a 0.45 μm pore size organic filter, and then concentrate to 100 μL with nitrogen. Store the concentrated samples in a -20°C refrigerator for later use.

[0023] S2. Identification of Compounds

[0024] The samples prepared in S1 were identified by gas chromatography-mass spectrometry (Model: Agilent 8890-5977B GC-MS), and the specific analysis conditions were as follows:

[0025] (1) Chromatographic cond...

Embodiment 2

[0030] Example 2: Screening of compounds with physiological activity on RIFA by GC-EAD experiment

[0031] GC-EAD was used to test the antennal electrophysiological responses of RIFA worker ants to compounds in the posterior thymus gland of P. strobilum to screen potential RIFA repellent active substances. The gas chromatographic model is Agilent 7890B, and the chromatographic conditions are: HP-5ms (30m, 0.25 mm inner diameter, 0.25 μm film thickness) capillary column, carrier gas is high-purity nitrogen, the inlet temperature is 250 °C, and the FID detector temperature is 320 °C , the oven heating program was 50 °C, the retention time was 2 min, then increased to 260 °C at 20 °C / min and held for 2 min. The EAG insect antenna potential recorder (Syntech, The Netherlands) was used to measure the electrophysiological responses of the thymus glands in the rear of P. The antennae were cut along the base of the antennae of the worker ants, and a small slit was cut at the tip of t...

Embodiment 3

[0033] Example 3: Repellent test of odor gland defense against RIFA

[0034] Ethovision XT11.5 software was used to record the repelling behavior characteristics of the thymus gland compounds against the RIFA workers. Place equilateral triangular filter paper sheets with a side length of 1 cm at two diagonal corners of a petri dish with a diameter of 9 cm, and divide the petri dish into two areas, the control area and the treatment area, with a vertical line intersecting the two pieces of filter paper. Drop 2 microliters of pure compound on a piece of filter paper, and the other piece of filter paper serves as a blank control ( Figure 4 (a)); 4 replicates for each compound; then put the RIFA worker ants in the center of the petri dish into the petri dish, and use the video software that comes with Ethovision XT to shoot and record the RIFA’s activity track in the petri dish5 min, and automatically count the activity time of RIFA in two different areas in the petri dish, usin...

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Abstract

The invention discloses application of hexanoic acid and hexyl hexanoate in repelling solenopsis invicta. The hexanoic acid and hexyl hexanoate which are screened and developed from the posterothorax stinky gland defensive compounds of giant limpridae have a relatively strong repelling effect on red imported fire ants, and can be used for development and quarantine application of behavior repellents of red imported fire ants and other ants; compared with other chemical repellents aiming at solenopsis invicta at the present stage, the repellent provided by the invention utilizes defensive substances naturally generated by insects, is greener and more environment-friendly, and has important market application and popularization values.

Description

technical field [0001] The invention belongs to the technical field of green prevention and control of RIFA, in particular to the application of hexanoic acid and hexyl hexanoate in repelling RIFA. Background technique [0002] In the long-term co-evolution of insects in nature with their natural enemies and niche competitors, various resistance mechanisms including physical, chemical and behavioral defenses have been formed to drive away the predation of natural enemies and the competition of other species of insects. Among them, chemical defense is the behavior of insects using chemical substances to drive away natural enemies and interfere with competitors. These chemical substances come from a wide range of sources, which may be directly from the host plants eaten by insects, their own gland secretions, or symbiotic bacteria in vivo. Therefore, the chemical defenses of insects have extremely important utilization value in the development of pest biological repellents. S...

Claims

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Application Information

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IPC IPC(8): A01N37/02A01N63/14A01P17/00
CPCA01N37/02A01N63/14Y02A50/30
Inventor 邱华龙徐金柱赖略秦长生杨华田龙艳彭龙春
Owner GUANGDONG ACAD OF FORESTRY
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