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SARS-CoV-2 neutralizing antibody kit and application thereof

A sars-cov-2 and kit technology, which is applied in the field of biomedical detection, can solve problems such as difficult quantitative detection of neutralizing antibody, and achieve the effect of improving detection sensitivity

Pending Publication Date: 2022-07-12
章文医药(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Currently, kits for detecting neutralizing antibodies against SARS-CoV-2 based on immunoassays can judge whether a sample contains neutralizing antibodies through the inhibition rate of ACE2 and RBD proteins, but it is difficult to quantitatively detect the amount of neutralizing antibodies in a sample

Method used

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  • SARS-CoV-2 neutralizing antibody kit and application thereof
  • SARS-CoV-2 neutralizing antibody kit and application thereof
  • SARS-CoV-2 neutralizing antibody kit and application thereof

Examples

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Embodiment 1

[0051] Basic Example 1. A SARS-CoV-2 Neutralizing Antibody Kit and Method of Use

[0052] 1. The protein expression vector pcDNA3.1(-) was purchased from Youbao Biological Company; the cloned strain DH5α competent cell (Item No.: TSV-A07) was purchased from Qingke Bio-Beijing Biotechnology Co., Ltd.; HEK293F for expression protein was purchased from ATCC cell bank; high-fidelity PCR enzyme (Cat. No. 10136ES08), purchased from Yisheng Bio; restriction endonucleases NheI (Cat. No.: FD0973) and NotI (Cat. No.: FD0594), purchased from Thermo; T4 DNA ligase (Cat. No.: FD0594) M0202S), purchased from NEB; 5μm fluorescent microspheres (Item No.: FM5CR03), purchased from thermo; Laibao Biotechnology Co., Ltd.), horseradish peroxidase (Luoyang Baiaotong Experimental Materials Center), and ELISA plate (Xiamen Yunpeng Technology Co., Ltd.).

[0053] 2. Main instruments: ETC811 PCR instrument (Suzhou Dongsheng Xingye Scientific Instrument Co., Ltd.), high-speed centrifuge (Xiangyi Labora...

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Abstract

The invention provides a flow-based SARS-CoV-2 neutralizing antibody kit and application thereof, and belongs to the field of biomedical detection. According to the present invention, the ACE2 receptor of the cell is simulated by coating the SARS-CoV-2 receptor ACE2 protein on the fluorescent microspheres, the virus infection is simulated by using the affinity of the trimer RBD protein and the ACE2, and the neutralizing antibody amount in the sample is quantitatively detected by determining the efficiency of the neutralizing antibody for inhibiting the combination of the ACE2 and the trimer RBD; therefore, the method can be used for judging the expression level of the neutralizing antibody after vaccination and evaluating the protective force of the vaccine.

Description

technical field [0001] The invention belongs to the field of biomedical detection, in particular to a flow-based SARS-CoV-2 neutralizing antibody kit and application thereof. Background technique [0002] Coronaviruses are a class of RNA viruses that can cause respiratory and intestinal infections in animals and humans. Among them, the pathogens of severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) and novel coronavirus pneumonia (COVID-19) are all. belongs to the coronavirus genus. According to the genome structure and phylogenetic analysis of coronaviruses, the family Coronaviridae can be divided into 4 genera: α, β, γ and δ. Coronaviruses of α and β genera generally infect mammals and humans, and coronaviruses of γ and δ genera generally infect mammals and humans. The virus mainly infects birds. Among them, SARS-CoV-2 is a new type of coronavirus of the β genus, round or oval in shape, about 60-140 nm in diameter, and has a crown-like s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/533
CPCG01N33/56983G01N33/533G01N2333/165
Inventor 章文羿程焕义黄清瑞尚雨寒南鹏娟巩惠琴赵会娟陈瀚
Owner 章文医药(北京)有限公司