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Preparation method and application of hippophae rhamnoides fruit extract for inhibiting xanthine oxidase

A technology of xanthine oxidase and extract, applied in the field of compound extraction, can solve the problem of less research on anti-hyperuricemia of seabuckthorn fruit, and achieve excellent xanthine oxidase inhibitory activity

Active Publication Date: 2022-07-22
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is less research on seabuckthorn fruit in anti-hyperuricemia

Method used

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  • Preparation method and application of hippophae rhamnoides fruit extract for inhibiting xanthine oxidase
  • Preparation method and application of hippophae rhamnoides fruit extract for inhibiting xanthine oxidase
  • Preparation method and application of hippophae rhamnoides fruit extract for inhibiting xanthine oxidase

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preparation example Construction

[0055] The invention provides a preparation method of a sea buckthorn extract with xanthine oxidase inhibitory activity. The specific preparation steps are as follows:

[0056] (1), the dried sea buckthorn fruit utilizes 80% ethanol extraction, suction filtration, collects the filtrate, and the filtrate is concentrated under reduced pressure to obtain the sea buckthorn ethanol extract; Extraction in turn to obtain petroleum ether extraction part (HF-PF), ethyl acetate extraction part (HF-EF) and n-butanol extraction part (HF-BF) respectively;

[0057] (2), the seabuckthorn ethyl acetate extraction part is separated by silica gel column, and uses CH 2 Cl 2 / MeOH stepwise elution yields six components Fr1-Fr7;

[0058] (3), use petroleum ether / ethyl acetate to separate Fr5 on a silica gel column to obtain eight components Fr5a to Fr5h; wherein Fr5g is purified by SephadexLH-20, eluted by reverse-phase ODS gradient, and then obtained by semi-preparative HPLC compound 1;

[00...

Embodiment

[0071] (1) The dried sea buckthorn fruit (10kg) was extracted with 3×20L of 80% ethanol at 80°C for 2h, the extract was filtered and concentrated under reduced pressure to obtain an ethanol extract; the extract was diluted with distilled water (5L), followed by Extract with petroleum ether (PE, 3×4.5L), ethyl acetate (EA, 3×4.5L) and n-butanol (n-Bu, 3×4.5L) to obtain 150 g of petroleum ether extract fraction (PF), respectively, Ethyl acetate extract fraction (EF) 80g and n-butanol extract fraction (BF) 1022g;

[0072] (2), Seabuckthorn ethyl acetate extract was separated by silica gel column (100-200 mesh), and used CH 2 Cl 2 / MeOH stepwise elution (100 / 1→50 / 1→20 / 1→10 / →8 / 10→6 / 1→4 / 1→2 / 1→1 / 1→MA) yields seven components (Fr 1-Fr7); take 2 mg of the three extracts respectively, dissolve with a small amount of DMSO, and then dilute to 10 mL with buffer to obtain 200 μg·mL -1 The seven components were tested for xanthine oxidase activity. The results are shown in Table 1. The ac...

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Abstract

The invention discloses a preparation method and application of a hippophae rhamnoides extract for inhibiting xanthine oxidase. Belongs to the technical field of compound extraction. The method comprises the following steps: 1, preparing a hippophae rhamnoides ethanol extract; 2, seven components including Fr 1, Fr 2, Fr 3, Fr 4, Fr 5, Fr 6 and Fr 7 are generated; 3, preparing a compound 1; 4, preparing compounds 2, 3 and 4; 5, preparing compounds 5, 6 and 7; by optimizing reaction conditions, effective components in sea-buckthorn are extracted to the greatest extent, and the extracted sea-buckthorn extract shows excellent xanthine oxidase inhibitory activity and has huge potential in the aspect of resisting hyperuricemia; the anti-xanthine oxidase contained in the sea-buckthorn has obvious activity; the theoretical guidance is provided for the application of the sea-buckthorn in the aspect of further functional food of the medicinal and edible plants.

Description

technical field [0001] The invention belongs to the technical field of compound extraction, and relates to a preparation method and application of a sea buckthorn extract inhibiting xanthine oxidase; in particular, to a sea buckthorn extract with xanthine oxidase inhibiting activity, a preparation method and application thereof. Background technique [0002] In the prior art, xanthine oxidase (XO) is a cytoplasmic enzyme present in various vertebrates including humans, belonging to the molybdenum flavonoid protein family, which is widely present in kidney, lung, heart and vascular endothelium. Xanthine oxidase is a key enzyme that controls the production of uric acid in some species, including humans. It controls the two key reactions of hypoxanthine to xanthine and xanthine to uric acid in purine metabolism in the human body. and the best activity in the intestine. Excessive activity of xanthine oxidase will lead to increased uric acid content in the body, resulting in hyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04C07D311/30C07D311/40C07H1/08C07H17/07C07D217/26A61K31/352A61K31/472A61K31/55A61K31/7048A61K36/185A61P19/06A23L33/105
CPCC07D487/04C07D311/30C07D311/40C07H1/08C07H17/07C07D217/26A61K36/185A61P19/06A23L33/105A61K2236/333A61K2236/35A61K2236/33A61K2236/53A23V2002/00A23V2200/30A23V2250/21A23V2250/2116Y02A50/30
Inventor 廖志新吕伸明
Owner SOUTHEAST UNIV
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