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A kind of xanthine oxidase inhibitor containing phenylalanine and its application

A xanthine oxidase and inhibitor technology, applied in the field of functional polypeptides, can solve problems such as reducing uric acid, liver and kidney damage, and achieve a good effect of xanthine oxidase inhibitory activity

Active Publication Date: 2021-08-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently available uric acid-lowering drugs have serious side effects such as liver and kidney damage to varying degrees.

Method used

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  • A kind of xanthine oxidase inhibitor containing phenylalanine and its application
  • A kind of xanthine oxidase inhibitor containing phenylalanine and its application
  • A kind of xanthine oxidase inhibitor containing phenylalanine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for extracting four kinds of polypeptides FH, FK, FVR and LFW in bonito meat, concrete steps are as follows:

[0030] (1) Protease hydrolysis of bonito meat: After crushing the bonito meat, add water and stir according to the mass ratio of 1:1, heat up to about 55°C, add trypsin with 1.0% of the bonito meat mass, enzymatically hydrolyze for 4 hours, then heat up to 95°C to kill the enzyme After 15 minutes, the enzymatic solution was concentrated by filtration and spray-dried to obtain bonito enzymatic hydrolyzate;

[0031] (2) Ethanol separation: Add 80% (V / V) ethanol to the bonito hydrolyzate at a mass ratio of 1:4, stir at 25°C for 1 hour, centrifuge at 4800×g for 15 minutes, and take the supernatant The alcohol-soluble component of bonito hydrolyzate was obtained after liquid concentration and spray drying;

[0032] (3) Gel column purification: redissolve the alcohol-soluble component of the bonito hydrolyzate with water to a concentration of 20% (W / V), a...

Embodiment 2

[0035] The solid-phase synthesis method of polypeptide FH, the specific steps are as follows:

[0036] Polypeptide FH was synthesized by a solid-phase polypeptide synthesis method, and the Fmoc (9-fluorenylmethoxycarbonyl) group was used as a polypeptide synthesis method for the α-amino protecting group—Fmoc method. First, the Fmoc-protected α-histidine (H) was covalently cross-linked to the resin, and then the protective agent was deprotected by alkaline solution and the carboxyl-activated α-phenylalanine (F) was cross-linked. FH, peptide Segments were finally quantitatively excised from the resin with TFA / dioxymethane. Purification was performed on a C18 reverse phase HPLC preparative column. Finally, the prepared polypeptide sample was rotary evaporated to remove residual organic reagents, etc., and after freeze-drying, it was made into a white powder, which was identified by mass spectrometer, and its purity was analyzed by reverse-phase HPLC. The purity requirement was g...

Embodiment 3

[0040] The solid-phase synthesis method of polypeptide FK, the specific steps are as follows:

[0041] Polypeptide FK is synthesized by a solid-phase polypeptide synthesis method, and the Fmoc (9-fluorenylmethoxycarbonyl) group is used as a polypeptide synthesis method for an α-amino protecting group—Fmoc method. First, the Fmoc-protected α-lysine (K) is covalently cross-linked to the resin, and then the protective agent is deprotected by alkaline solution and the carboxyl-activated α-phenylalanine (F) is cross-linked with FK, and the peptide Finally, it was quantitatively cleaved from the resin with TFA / dioxymethane. Purification was performed on a C18 reverse phase HPLC preparative column. Finally, the prepared polypeptide sample was rotary evaporated to remove residual organic reagents, etc., and after freeze-drying, it was made into a white powder, which was identified by mass spectrometer, and its purity was analyzed by reverse-phase HPLC. The purity requirement was grea...

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Abstract

The invention discloses a xanthine oxidase inhibitor containing phenylalanine and its application in the preparation of medicines for preventing and treating hyperuricemia and gout. The inhibitor contains polypeptide FH, FK, FVR or LFW More than one of them; these four polypeptides can be separated from the bonito protein enzymatic hydrolyzate, add ethanol to the bonito enzymatic hydrolyzate, centrifuge to take the supernatant, concentrate and spray dry to obtain the alcohol-soluble component of the bonito enzymatic hydrolyzate; The components were separated by Sephadex G-15, and finally separated by UPLC to obtain the four polypeptides. The four polypeptides of the present invention can be chemically synthesized, and can also be directional prepared from bonito hydrolyzate, and have good xanthine oxidase inhibitory activity, so they can be modified directly or as lead compounds to be used in the prevention and treatment of In health food or medicine for treating hyperuricemia and gout.

Description

technical field [0001] The invention belongs to the field of functional polypeptides, in particular to a xanthine oxidase inhibitor containing phenylalanine and its application in medicines for preventing and treating hyperuricemia and gout. Background technique [0002] Hyperuricemia is a syndrome caused by abnormal production and / or excretion of uric acid in the human body. It is also the cause of many chronic diseases such as gout, chronic kidney disease, heart failure, stroke, and atherosclerosis. The main feature is that the uric acid content in plasma exceeds the normal range (more than 7.0mg / mL for men and more than 6.0mg / mL for women). [0003] In the human body, xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine, and further catalyzes the transformation of xanthine into uric acid, so it is an oxidase necessary for the production of uric acid in the human body. Studies have found that xanthine oxidase inhibitors (allopurinol, febuxostat, polyphenol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/065C07K5/087C07K5/083C12P21/06C07K1/14C07K1/16A61K38/05A61K38/06A61P19/06
CPCA61K38/00A61P19/06C07K5/06078C07K5/0808C07K5/0812C12P21/06
Inventor 苏国万何伟炜赵谋明孙东晓
Owner SOUTH CHINA UNIV OF TECH
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