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Process for inhibiting xanthine oxidase activity in vitro by using alkalized catechin monomer

A technology of xanthine oxidase and catechins, which is applied in the field of inhibiting xanthine oxidase activity in vitro by alkalized catechin monomers, can solve the problems of no inhibitory effect, and improve the inhibitory effect and source of raw materials Extensive, easy-to-use effects

Pending Publication Date: 2022-08-05
TONGREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, we have found that catechin monomers have no inhibitory effect on the activity of xanthine oxidase through exploration, and using this method to treat catechin monomers can largely inhibit xanthine oxidase (XOD) Activity

Method used

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  • Process for inhibiting xanthine oxidase activity in vitro by using alkalized catechin monomer
  • Process for inhibiting xanthine oxidase activity in vitro by using alkalized catechin monomer
  • Process for inhibiting xanthine oxidase activity in vitro by using alkalized catechin monomer

Examples

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Effect test

Embodiment 1

[0041] A process for inhibiting xanthine oxidase activity in vitro by utilizing alkalized catechin monomers, comprising the following steps:

[0042] S1. Material preparation: prepare a sodium pyrophosphate buffer with a pH of 7.5, accurately weigh 2.659 g of sodium pyrophosphate, and 0.0175 g of EDTA by ultrasonically dissolving it into purified water, set the volume to 100 mL, and adjust the pH to 7.5 with phosphoric acid to obtain 0.1mol / L sodium pyrophosphate buffer, ready-to-use;

[0043] Dissolve the xanthine oxidase solid in the 0.1mol / L sodium pyrophosphate buffer solution to configure a 7.2u / mL xanthine oxidase mother solution, and then dilute it into a 30mu / mL xanthine oxidase solution; dissolve the xanthine solid Prepare 1 mmol / L xanthine stock solution in purified water, then dilute it to 0.3 mmol / L xanthine solution, store in the dark, dissolve epicatechin in purified water to prepare 1500 μmol / L solution, and then dilute to 750 μmol / L of catechin monomer diluen...

Embodiment 2

[0047] A process for inhibiting xanthine oxidase activity in vitro by utilizing alkalized catechin monomers, comprising the following steps:

[0048] S1. Material preparation: prepare a sodium pyrophosphate buffer with a pH of 7.5, accurately weigh 2.659 g of sodium pyrophosphate, and 0.0175 g of EDTA by ultrasonically dissolving it into purified water, set the volume to 100 mL, and adjust the pH to 7.5 with phosphoric acid to obtain 0.1mol / L sodium pyrophosphate buffer, ready-to-use;

[0049] Dissolve the xanthine oxidase solid in the 0.1mol / L sodium pyrophosphate buffer solution to configure a 7.2u / mL xanthine oxidase mother solution, and then dilute it into a 30mu / mL xanthine oxidase solution; dissolve the xanthine solid Prepare 1 mmol / L xanthine stock solution in purified water, then dilute it to 0.3 mmol / L xanthine solution, store in the dark, dissolve epicatechin in purified water to prepare 1500 μmol / L solution, and then dilute to 750 μmol / L of catechin monomer diluen...

Embodiment 3

[0053] A process for inhibiting xanthine oxidase activity in vitro by utilizing alkalized catechin monomers, comprising the following steps:

[0054] S1. Material preparation: prepare a sodium pyrophosphate buffer with a pH of 7.5, accurately weigh 2.659 g of sodium pyrophosphate, and 0.0175 g of EDTA by ultrasonically dissolving it into purified water, set the volume to 100 mL, and adjust the pH to 7.5 with phosphoric acid to obtain 0.1mol / L sodium pyrophosphate buffer, ready-to-use;

[0055] Dissolve the xanthine oxidase solid in the 0.1mol / L sodium pyrophosphate buffer solution to configure a 7.2u / mL xanthine oxidase mother solution, and then dilute it into a 30mu / mL xanthine oxidase solution; dissolve the xanthine solid Prepare 1 mmol / L xanthine stock solution in purified water, then dilute it to 0.3 mmol / L xanthine solution, store in the dark, dissolve epicatechin in purified water to prepare 1500 μmol / L solution, and then dilute to 750 μmol / L of catechin monomer diluen...

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Abstract

The invention discloses a process for inhibiting the activity of xanthine oxidase in vitro by using an alkalized catechin monomer, and belongs to the technical field of catechins, and the process comprises the following steps: S1, preparing materials: preparing a sodium pyrophosphate buffer solution with the pH value of 7.5, and preparing a xanthine oxidase mother solution, a xanthine solution and a catechin monomer diluent for later use; s2, alkalizing catechin monomers: adding an alkaline solution into the catechin monomer diluent, and reacting in a water bath kettle at 35 DEG C for 30 minutes to obtain alkalized catechin monomers; s3, taking a mixture of the xanthine oxidase solution, the xanthine solution and the buffer solution as a solvent I, mixing the solvent I with the alkalized catechin monomer by adopting a compatible solvent method, and reacting in a water bath kettle at 35 DEG C for 30 minutes, so as to inhibit the activity of the xanthine oxidase in vitro; the catechin monomers are subjected to alkalization treatment, so that the alkalized catechin monomers have an inhibition effect on the activity of xanthine oxidase.

Description

technical field [0001] The invention relates to the technical field of catechins, in particular to a process for inhibiting xanthine oxidase activity in vitro by using alkalized catechin monomers. Background technique [0002] Tea contains a variety of components, the main components of which are tea polyphenols, alkaloids, sugars, amino acids and so on. The main component of tea polyphenols is catechin, which is its active ingredient. Catechin, also known as tea tannin and catechin, is a class of flavan-3-ol derivatives. It is a white solid with bitter, astringent and astringent properties. , has strong hydrophilicity, and catechins in tea leaves are stored in large amounts in the new shoots of tea trees, accounting for 12%-24% of the dry weight of tea leaves, and its representative catechins include epicatechin (EC) , epigallocatechin (EGC), epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and the like. Its health care function: it has antioxidant, anti-tumor, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P19/06
CPCA61K31/353A61P19/06
Inventor 陈仕学龙洋姚元勇陈双冯影杨长远潘婷
Owner TONGREN UNIV
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