Intracellular isoform of the interleukin-1 receptor antagonist

An interleukin and antagonistic technology, applied in the field of biology, can solve the problems of unclear biological significance

Inactive Publication Date: 2005-10-26
LAB SERONO SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological significance of icIL-1ra remains unclear

Method used

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  • Intracellular isoform of the interleukin-1 receptor antagonist
  • Intracellular isoform of the interleukin-1 receptor antagonist
  • Intracellular isoform of the interleukin-1 receptor antagonist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Cloning and Characterization of icIL-1ra II

[0068] Materials and methods

[0069] Reagent

[0070] The following commercially available reagents are used for cell culture and isolation: pyrogen-free saline and distilled water for clinical use: RPMI 1640 medium; DMEM medium; M199 medium; L-glutamine; Percoll; Ficoll-Hipaque; Aseptically collected fetal bovine serum; Endothelial cell growth supplement (ECGS) prepared from bovine brain; Heparin.

[0071] The endotoxin contained in all reagents is less than 0.125EU / ml, which is checked by limulus amebocyte lysate test.

[0072] cell

[0073] Human circulating PMN and mononuclear cells are separated by isotonic (285mOsm) Perke discontinuous (46% for monocytes and 62% for PMN) gradient centrifugation from the peripheral blood of healthy donors, such as Colotta F., Peri G., The method described in Villa SA., Mantovani A., Rapid killing of actinomycin D treated tumor cells by human mononuclear cells. J. Immunol. 132:936,1...

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Abstract

This invention discloses a new interleukin-1 antagonist active both against IL-1 alpha and IL-1 beta , a new DNA sequence encoding the Il-1 antagonist and the method for obtaining a IL-1 antagonist by the recombinant DNA technique; it is also described the prophylactic, therapeutic and diagnostic use of such new IL-1 antagonist in pathologies deriving from the IL-1 production.

Description

technical field [0001] The present invention belongs to the field of biotechnology. It describes a novel interleukin-1 (IL-1) antagonist active against both IL-1a and IL-1B, a novel DNA sequence encoding an IL-1 antagonist and recombinant DNA technology Process for obtaining IL-1 antagonists. It also describes the use of this novel IL-1 antagonist in the prophylaxis, treatment and diagnosis of pathologies due to IL-1 production. Background technique [0002] There are two distinct interleukin-1-encoding genes named IL-1a and IL-1B, which encode IL-1a protein and IL-1B protein, respectively. [0003] Interleukins IL-1a and IL-1B are pleiotropic cytokines, although they show little similarity in sequence, they are effective in different tissues and in many human pathologies, especially in the immune response of tissues and The inflammatory process produces similar effects. [0004] Both proteins have a molecular weight of approximately 17.5 KDa and have both been previousl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09A61K38/00A61K38/20A61P7/06A61P29/00A61P31/04A61P35/02A61P37/00C07K5/083C07K14/54C12N5/10C12N15/12C12N15/24C12N15/25C12N15/85
CPCC07K14/54A61K38/00A61P1/04A61P19/02A61P29/00A61P31/04A61P31/18A61P35/00A61P35/02A61P37/00A61P37/06A61P7/06C12N15/11C12N5/10A61K38/20
Inventor 弗朗切斯科·科洛塔马尔塔·穆齐奥阿尔贝托·曼托瓦尼
Owner LAB SERONO SA
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