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Hydrophilic poly methyl methacrylate chip material, chip and its preparing method

A polymethyl methacrylate and chip material technology, which is applied in the field of micro-total analysis, can solve the problems of shedding of hydrophilic groups, complicated modification process, influence on repeatability, etc., and achieves improved hydrophilicity, simple process, and improved separation. degree of effect

Inactive Publication Date: 2006-07-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage is that the hydrophilicity can be greatly improved, but the disadvantage is that after repeated use of the chip, the hydrophilic group will easily fall off, and the hydrophilicity will decrease, which will seriously affect the repeatability of its use.
In addition, the modification process is complicated and takes a long time (more than several hours)

Method used

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  • Hydrophilic poly methyl methacrylate chip material, chip and its preparing method
  • Hydrophilic poly methyl methacrylate chip material, chip and its preparing method
  • Hydrophilic poly methyl methacrylate chip material, chip and its preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: φX-174 / HaeЩ digest DNA standard was separated on an unmodified PMMA chip.

[0016] Polymethyl methacrylate microfluidic chips were fabricated by conventional methods.

[0017] The φX-174 / HaeЩ digest DNA standard is separated on this PMMA chip: the standard concentration is 0.5ng / μL (142pM). The separation conditions were 1.5% HPMC in 100mM Tris / 100mM boric acid / 2mM EDTA, the dye concentration was 1μM SYTOX Orange, the separation length was 40mm, and the separation voltage was 270V / cm. The injection volume was 177pL. The separation profile of φX-174 / HaeЩ digest DNA standard on the unmodified PMMA chip is as follows: figure 1 shown.

Embodiment 2

[0018] Example 2: φX-174 / HaeЩ digest DNA standard was separated on the PMMA chip modified by the patented method.

[0019] Adopt a kind of organic matter ABS plastic (acrylonitrile-butadiene-styrene Acrylonitrile Butadiene Styrene) with good hydrophilicity to add ABS plastic in PMMA before making polymethyl methacrylate microfluidic chip, make in the raw material The content of ABS plastic is 5%, and then injection molded into chips according to conventional methods.

[0020] The φX-174 / HaeЩ digest DNA standard is separated on the PMMA chip modified by this patented method: the concentration of the standard is 0.5ng / μL (142pM). The separation conditions were 1.5% HPMC in 100mM Tris / 100mM boric acid / 2mM EDTA, the dye concentration was 1μM SYTOX Orange, the separation length was 40mm, and the separation voltage was 270V / cm. The injection volume was 177pL. The separation profile of φX-174 / HaeЩ digest DNA standard on the modified PMMA chip is as follows: figure 2 shown.

[002...

Embodiment 3

[0023] Example 3: Detection of the fluorescent dye rhodamine 6G by using the modified PMMA chip of this patent.

[0024] A polymethyl methacrylate microfluidic chip was fabricated according to Example 2.

[0025] Utilize this modified PMMA chip to detect the minimum detection limit chip electrophoresis pattern of fluorescent dye rhodamine 6G as follows image 3 shown.

[0026] In terms of detection sensitivity, under the same conditions to detect the fluorescent dye rhodamine 6G, the sensitivity of this embodiment can reach 6.67 × 10 -13 mol / L, while the detection sensitivity of ordinary polymethyl methacrylate microfluidic chip is 1.0×10 -10 mol / L, the sensitivity of this embodiment is improved by more than 100 times in comparison.

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Abstract

The present invention provides hydrophilic polymethyl methacrylate micro flow controlling chip material, the chip prepared with the material and its preparation process. The material is composition of polymethyl methacrylate and hydrophilic chemical matter. The chip of the present invention has the advantages of overall denaturation of the material, raised and maintained chip hydrophilicity, no influence of physical and chemical factors and reusability of the chip. The present invention has the physical and chemical advantages of obviously raised hydrophilicity, obviously increased electric percolation; raised DNA separating degree, and 100 times over raised detection sensitivity. The present invention has simple preparation process and may be used in industrial production.

Description

Technical field: [0001] The invention relates to micro-total analysis technology, and in particular provides a hydrophilic chip material, a chip and a preparation method thereof. Background technique: [0002] The modification method reported in the document "Lin, Y.J., Ganser, D., Schneider, A., et al. Microfabricated polycarbonate CE devices for DNA analysis. Anal. Chem. 2001, 73, 4196-4201" is to polycarbonate the chip material The ester surface was exposed to UV light for up to 5 hours to increase the hydrophilicity of the surface. The disadvantage is that the processing time is long, and the material's high temperature or chemical treatment may lead to changes in surface chemical properties, and its hydrophilicity will decrease, which will affect its repeatability. [0003] Literature "Li, X., Regnier, F.E.Channel-specific coatings onmicrofabricated chips.J.Chromatogra.A, 2001, 924, 165-176" and "Soper, S.T., Henry, A.C., Vaidya, B., et al.Surface The modification of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L33/12G01N33/53
Inventor 周小棉戴忠鹏罗勇林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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