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Tissue culture method for fast propagation of fragrant shaddock

A tissue culture rapid propagation and fragrant pomelo technology is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as the uniformity of variation of sexually propagated plants, and achieves well-developed root system of tissue culture seedlings and good plant growth. Effect

Inactive Publication Date: 2002-10-16
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a method for rapid propagation of pomelo, overcome the shortcomings of sexually propagated plant variation and poor uniformity, and achieve fast access to excellent pomelo seedlings with neat and consistent plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 (is treatment No. 1). Soak the seeds in warm water at 45°C for 5 minutes, and then soak them in hot water at 60°C for 55 minutes. The seeds were sterilized by 15% sodium hypochlorite for 20 minutes, and the seeds were inoculated in aseptic medium MS1 (the percentage by weight is MS inorganic salt 0.44%, sucrose 1.5%, 6BA 1×10 -6 , NAA 2×10 -7 , plant gel 0.25%), placed in 27 DEG C under dark conditions for cultivation; the cultivated seeds were replaced with a medium every 10 days; after 25 days of cultivation, the seeds germinated into green shoots. Get the expanded green cotyledon inoculation medium MS2 (weight percentage is MS inorganic salt 0.44%, sucrose 1.5%, NAA 2 * 10 -7 , 6BA 1×10 -6 , biotin 1 x 10 -7 , Folic Acid 1 x 10 -7 , Riboflavin 1×10 -7 , vitamin C 5 x 10 -6 , plant gel 0.25%), cultivated into cluster buds through 25 days; the cluster shoot tissue that grows into about 1.5cm diameter is cut into the fritter that every piece diameter is...

Embodiment 2

[0059] Embodiment 2 (is No. 2 processing), seed is soaked 10min in 45 ℃ of warm waters, soaks 50min with 55 ℃ of hot waters again, seed is sterilized 15min with 15% sodium hypochlorite, the substratum MS1 that seed is inoculated in sterilization (weight percent is MS Inorganic salt 0.44%, sucrose 1.5%, 6BA 1×10 -6 , NAA 2×10 -7 , plant gel 0.25%), placed in 27 DEG C under dark conditions for cultivation; for the cultured seeds, the medium was replaced every 10 days; after 20 days of cultivation, the seeds germinated into green shoots. Get the expanded green cotyledon inoculation medium MS2 (weight percentage is MS inorganic salt 0.44%, sucrose 1.5%, NAA 1.5 * 10 -7 , 6BA 1×10 -6 , biotin 1 x 10 -7 , Folic Acid 1 x 10 -7 , Riboflavin 1×10 -7 , vitamin C 3 x 10 -6 , plant gel 0.25%), cultivated into cluster buds through 30 days; the cluster shoot tissue that will grow into about 1.5cm in diameter is cut into the fritter that every piece of diameter is 0.3cm, inoculates in ...

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PUM

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Abstract

The present invention is one kind of tissue culture and fast propagation technology for fragrant shaddock. By means of material screening, culture medium component improvement, hormone regulation andother technological means, tissue culture rate of 1000 to 10000 times each year is reached. The present invention makes it possible to provide large amount of fragrant shaddock seedlings in high quality in greening city.

Description

technical field [0001] The invention belongs to the technical field of biological group cultivation seedlings, and in particular relates to a method for rapid propagation of pomelo through tissue culture. Background technique [0002] With the development of society and the improvement of people's living standards, the optimization and beautification of urban ecology and road environment has become an important issue in urban development. Selecting high-quality tree species to plant on both sides of the road to form an ecological forest has become an indispensable part of this issue. . European and American countries pay great attention to the selection of different tree species and combinations according to the characteristics of different cities, such as symbolic national flowers and city flowers (cherry blossoms, red maple, loblolly pine, etc.), and special ornamental tree species (such as araucaria, betel nut, oil palm, etc.) , There are also trees (pine, cypress, rue, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 明凤叶鸣明沈大棱
Owner FUDAN UNIV
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