A kind of plant tissue culture rapid propagation method using shoot tip as explant

A plant tissue culture and explant technology, applied in the field of fast propagation of plant tissue culture, can solve the problems of "ling seed" easy senescence, long plant breeding time, unstable germplasm resources, etc. Short, fast-reproducing effects
CN112931220BActive Publication Date: 2022-05-27INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
Publication Date
2022-05-27

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Abstract

The invention discloses a plant tissue culture rapid propagation method using stem tips as explants: (1) disinfection treatment of explants: take the stem tips on plants as explants and disinfect the surface; the plants are Rhizoma Chuanxiong or bean (2) rooting culture: transfer the sterilized stem tip to the rooting medium, and cultivate it at 24-26°C, 12-16 hours of light per day, and light intensity of 1000-1500Lx; (3) Seedling hardening and transplanting: When the adventitious roots regenerated at the base of the shoot tip grow to about 1-4 cm in rooting medium, open the bottle cap, harden the seedlings indoors, and transplant them into the substrate for planting. The shoot tip tissue rapid propagation technology of the present invention directly induces the shoot tip to regenerate adventitious roots in vitro, without the steps of callus induction and differentiation culture, eliminating the cumbersome process of preparing various culture media and rotating bottles, and can rapidly propagate plants. The traditional tissue culture rapid propagation method can shorten the tissue culture cycle by 1 to 2 months.
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Description

technical field

[0001] The invention relates to the technical field of rapid propagation of plant tissue culture, in particular to a method for rapid propagation of plant tissue culture using shoot tips as explants. Background technique

[0002] Chuanxiong (scientific name: Ligusticum chuanxiong hort) is one of the most common Chinese herbal medicines in my country, and it is also an important raw material for many health products and medicines. Recently, Chuanxiong has become a star Chinese herbal medicine in the fight against the new crown (COVID-19) epidemic. "Xuebijing Injection", which uses safflower, red peony, chuanxiong, salvia, and angelica as the main raw materials, has been approved for the treatment of severe novel coronavirus pneumonia. , critical systemic inflammatory response syndrome and multiple organ failure.

[0003] Chuanxiong is a biennial plant, and it is difficult for Chuanxiong to bear fruit. The traditional breeding method of Chuanxiong is to use st...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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