Plant tissue culture rapid propagation method taking stem tip as explant

A plant tissue culture and explant technology, applied in the field of fast propagation of plant tissue culture, can solve the problems of unstable germplasm resources, long plant breeding time, and easy aging of "ling seeds", and achieve rapid propagation and tissue culture cycle. Short, well-rooted effect

Active Publication Date: 2021-06-11
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of stem plant for the long time of plant breeding in the prior art, unstable germplasm resources, "Lingzi" itself is easy to age and carry bacteria, and the traditional tissue culture fast propagation cycle is long and easy to be polluted. The plant tissue culture rapid propagation method with the tip of the explant, compared with the existing tissue culture rapid propagation system, this method can save 1 to 2 months of tissue culture cycle, and a large number of rapid growth in a short period of time is fast and the quality is excellent and tissue culture seedlings with low infection rate

Method used

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  • Plant tissue culture rapid propagation method taking stem tip as explant
  • Plant tissue culture rapid propagation method taking stem tip as explant
  • Plant tissue culture rapid propagation method taking stem tip as explant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the influence of different hormone ratios on the shoot tip regeneration of Rhizoma Chuanxiong

[0035] (1) Material collection: The young stem tips of Rhizoma Chuanxiong at the mature stage, about 2 cm to 4 cm in length, are directly picked as explants, without the need to peel off young leaves and leaf primordia or cut out the shoot tips with dissecting needles under a dissecting microscope. In the prior art, the stem tip of Rhizoma Chuanxiong is used as the explant to propagate and cultivate. The stem tip explant of Rhizoma Chuanxiong with 1 to 2 true leaves needs to be peeled off the shoot tip meristem under a microscope, and 2-3 mm The explants are then propagated through callus induction and differentiation culture, which is a cumbersome process and takes a long time.

[0036] (2) Disinfection treatment of explants: Rinse the stem tip of Rhizoma Chuanxiong carefully under running water for 1 hour, then wash it 3 times with sterile water in an ultra-cl...

Embodiment 2

[0050] Embodiment 2, Rhizoma Chuanxiong stem tip tissue culture rapid propagation method

[0051] According to the optimal rooting medium of the shoot apex screened in the previous stage, the method of tissue culture and rapid propagation of the shoot apex of Ligusticum chuanxiong was established by using the direct organogenesis method. The operation steps are as follows:

[0052] (1) Material collection: select young shoot tips of Rhizoma Chuanxiong in the mature stage, about 2 cm to 4 cm in length, as explants.

[0053] (2) Disinfection treatment of explants: Rinse the stem tip of Rhizoma Chuanxiong carefully under running water for 1-2 hours, then wash it with sterile water for 3 times in an ultra-clean bench, then treat it with 70% or 75% alcohol for 30 seconds, and then wash it with 2 % (W / V) sodium hypochlorite solution for 4 minutes, and finally washed 3 times with sterile water.

[0054] (3) Stem tip rooting medium: Transfer the sterilized Rhizoma Chuanxiong stem tip...

Embodiment 3

[0057] Embodiment 3, Ligusticum chuanxiong stem node tissue culture rapid propagation system

[0058] Learn from the tissue culture and rapid propagation system of Rhizoma Chuanxiong stem tip, and use the best rooting medium screened in the previous stage to establish the tissue culture and rapid propagation system of Rhizoma Chuanxiong stem nodes. The operation steps are as follows:

[0059] (1) The explants of Rhizoma Chuanxiong stem nodes near the tip of the stem were selected, and the stem nodes near the underground part were difficult to sterilize and had a high pollution rate.

[0060] (2) Disinfect and sterilize the stem node explants of Rhizoma Chuanxiong. The disinfection method adopted is: first rinse the stem nodes under running water for about 3 hours, then treat them with 75% alcohol for 45 seconds and 2% sodium hypochlorite for 8 minutes under sterile conditions, and finally wash the explants with sterile water for 3-3 hours. 5 times.

[0061] (3) Transfer the ...

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Abstract

The invention discloses a plant tissue culture rapid propagation method taking a stem tip as an explant. The method comprises the following steps of (1) disinfecting the explant: taking the stem tip on a plant as the explant, and carrying out surface disinfection, wherein the plant is Ligusticum chuanxiong hort or Pachyrhizus erosus or Psophocarpustetragonolobus; (2) performing rooting culture: transferring the disinfected stem tip to a rooting culture medium, and culturing the stem tip under the conditions that the temperature is 24-26 DEG C, the illumination time is 12-16 hours every day and the illumination intensity is 1000-1500 Lx; and (3) performing seedling hardening and transplanting: when the stem tip is cultured in the rooting culture medium till the regenerated adventitious root at the base part of the stem tip is about 1-4 cm, opening a bottle cap, and transplanting the stem tip into a matrix for planting after seedling hardening indoors. According to the stem tip tissue rapid propagation technology, the stem tip is directly induced to regenerate the adventitious root in vitro, the steps of callus induction, differentiation culture and the like are not needed, the tedious processes of preparing multiple culture media and performing bottle transfer are omitted, plants can be rapidly propagated, and compared with a traditional tissue culture rapid propagation method, the method provided by the invention has the advantage that the tissue culture period can be shortened by 1-2 months.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a plant tissue culture rapid propagation method using shoot tips as explants. Background technique [0002] Chuanxiong (scientific name: Ligusticum chuanxiong hort) is one of the most common Chinese herbal medicines in my country, and it is also an important raw material for many health products and medicines. Recently, Chuanxiong has become a star Chinese herbal medicine in the fight against COVID-19. "Xuebijing Injection" with safflower, red peony root, Chuanxiong, Danshen and Angelica as main raw materials has been approved for the treatment of severe cases of novel coronavirus pneumonia , critical systemic inflammatory response syndrome and multiple organ failure. [0003] Rhizoma Chuanxiong is a biennial plant, and it is difficult for Rhizoma Chuanxiong to bear fruit. The traditional breeding method of Rhizoma Chuanxiong is to use stem node ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 祝婷婷任茂智李林宣方琳
Owner INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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