Slow-releasing bFGF-PLGA microball and its prepn and use

A microsphere and slow-release technology, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., and can solve the problems that there are no bFGF microspheres.

Inactive Publication Date: 2003-02-26
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Slow-releasing bFGF-PLGA microball and its prepn and use
  • Slow-releasing bFGF-PLGA microball and its prepn and use
  • Slow-releasing bFGF-PLGA microball and its prepn and use

Examples

Experimental program
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Embodiment Construction

[0013] Take 150 mg of PLGA and other auxiliary materials and dissolve it in an organic solvent to make an oil phase. The organic solvent is dichloromethane (DCM) or a mixture of dichloromethane (DCM) and acetone (AC), and the volume percentage of DCM and AC is 75-100:0-35. A 100 μg sample of bFGF was dissolved in double distilled water to form an aqueous phase. The bFGF solution is added to the above-mentioned oil phase and homogenized to form colostrum. Weigh an appropriate amount of PVA or PVA+PEG into double distilled water, make it fully dissolved in a water bath at 50°C, and adjust its concentration to 6%. Inorganic salt is added to the aqueous dispersion medium solution so that the concentration of the outer aqueous phase is 2% or higher. Add the above emulsion dropwise to 2ml of PVA or PVA-PEG solution, homogenize again, pour this solution into 8ml of the same concentration of PVA or PVA-PEG solution, and magnetically stir for 3-5 hours at room temperature to obtain bFGF-PL...

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Abstract

The present invention provides one kind of slow-releasing basic fibroblast growdth factor (bFGF) microsphere and its preparation and use. The microsphere has coated bFGF as medicine, and matrix of polylactide-co-glycolide (PLGa). The slow-releasing bFGF microsphere is prepared by using PVA or mixed PVA-PEG liquid as dispersing medium and through w/o/wt% re-emulsion and drying process and mechanical stirring. Its freeze dried power has good dispersivity and low organic solvent residue. The present invention can be used for local administration or vein administration of treating fracture, bone defect and other diseases.

Description

Technical field [0001] The invention relates to a sustained-release microsphere of basic fibroblast growth factor (bFGF). The preparation method is a w / o / w double emulsion-drying method, using a polymer material PLGA as a carrier material to encapsulate bFGF, and preparing the sustained-release bFGF microspheres under the action of mechanical stirring. Background technique [0002] Basic fibroblast growth factor (bFGF) is not only a mitogen, but also the strongest angiogenic growth factor known in the body. As a broad-spectrum mitogen, bFGF can promote the proliferation and differentiation of a variety of cells such as mesenchymal cells, pre-osteoblasts, osteoblasts, chondrocytes, Schwann cells and fibroblasts. Studies have shown that exogenous bFGF can promote a variety of cell division and chemotaxis, induce normal embryo development, promote blood vessel growth, wound healing, nerve regeneration and functional recovery. [0003] Since the half-life of bFGF in the body is 3-5 m...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K9/52A61K38/18A61P19/08
Inventor 裴福兴段宏沈彬陈坚王光林何勤
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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