Preparation method and use of skunk bush extract morroniside

A technology of Cornus officinalis and its extract, which is applied in the field of preparation of Cornus officinalis extract-morroniside, a monomer compound, can solve the problems of the preparation method of Cornus officinalis-morroniside and the prevention and treatment of diabetic vascular diseases, etc., and achieve improvement The morphological and pathological changes of vascular endothelium and kidney tissue, the increase of protein excretion rate, and the effect of simple and feasible preparation method

Inactive Publication Date: 2005-01-26
江苏中康药物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, so far, there has been no report on the preparation method of cornus officinalis extract-morroniside for pharmaceutical use and the prevention and treatment of diabetic vascular disease

Method used

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  • Preparation method and use of skunk bush extract morroniside

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0179] 1000 g of Cornus officinalis medicinal material is reflux extracted with 60% ethanol for 3 times, the amount of alcohol added is 15 times of the weight of medicinal material, and the extraction time is 120 minutes. Combine the extracts, filter and concentrate under reduced pressure, add 4 times the amount of water to the concentrated solution for precipitation and centrifuge, use activated carbon for column chromatography on the supernatant, wash away impurities with water and 5% ethanol, and then use 50-70% ethanol Gradient elution, collect the ethanol eluent, dry it under reduced pressure, and perform reverse phase silica gel column chromatography, use 30%-60% ethanol solution for gradient elution, combine the same fractions through thin layer detection, add 40%-60% ethanol The eluate of the solution was dried under reduced pressure to obtain morroniside, the extract of Cornus officinalis in powder form.

Embodiment 2

[0181] 1000 g of Cornus officinalis medicinal material is reflux extracted with 80% ethanol for 3 times, the amount of alcohol added is 12 times of the weight of medicinal material, and the extraction time is 150 minutes. Combine the extracts, filter and concentrate under reduced pressure, add 5 times the amount of water to the concentrate and centrifuge, use macroporous resin for column chromatography on the supernatant, wash away impurities with water and 10% ethanol, and then use 30~ Gradient elution with 50% ethanol, collect the ethanol eluate, dry under reduced pressure, perform reverse phase silica gel column chromatography, use gradient elution with 20% to 50% ethanol solution, combine the same fractions through thin layer detection, and combine 30% to 50% ethanol The eluate of 50% ethanol solution was dried under reduced pressure to obtain morroniside, the extract of Cornus officinalis in powder form.

Embodiment 3

[0183] 1000 g of the medicinal material of Cornus officinalis is crushed and passed through a 40-mesh sieve, impregnated with 40% ethanol for 36 hours, and then extracted by percolation at a flow rate of 4-5 ml / min, and the percolation amount is 10 times the weight of the medicinal material. Combine the extracts, filter and concentrate under reduced pressure, add 4 times the amount of water to the concentrated solution to precipitate and centrifuge, use macroporous adsorption resin for column chromatography on the supernatant, wash away impurities with water and 5% ethanol, and then use 30~ 50% ethanol gradient elution, collect the ethanol eluate, dry it under reduced pressure and perform reverse-phase silica gel column chromatography, use 20-50% ethanol solution gradient elution, combine the same fractions through thin layer detection, and combine 30%- The eluate of 50% ethanol solution was dried under reduced pressure to obtain morroniside, the extract of Cornus officinalis i...

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Abstract

The invention discloses a preparation method and use of skunk bush extract morroniside which consists of, extracting the Cornel medicinal material with ethanol, decompression concentration, concentration alcohol depositing, subjecting the supernatant fluid to column chromatography with macroporous absorption resin or activated charcoal, removing the impurity substance, gradient ethanol elution, decompressing, concentrating and drying the eluent, and reversed phase silica gel segregation. The invention also discloses its use in preparation of drug for preventing and treating diabetic nephropathy.

Description

technical field [0001] The invention relates to a preparation method and application of a monomer compound morroniside, which is an extract of cornus officinalis, and belongs to the field of traditional Chinese medicine. Background technique [0002] Diabetes mellitus is a chronic systemic progressive endocrine and metabolic disease. Sustained high blood sugar can lead to abnormal metabolism of some tissues and organs, and then cause their dysfunction and morphological changes. The duration of diabetes is more than 10 years, and most patients are complicated with vascular complications of varying degrees, especially microvascular complications. Diabetic microangiopathy refers to microcirculatory disturbance, microangioma formation and thickening of microvascular basement membrane, mainly including diabetic nephropathy and diabetic retinopathy, which can lead to uremia or blindness in severe cases, causing great physical and mental pain to patients. Therefore, it is of great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P3/10A61P9/00A61P13/12A61P27/02C07H1/08C07H17/04
Inventor 肖伟潘扬丁岗章晨峰韩淑燕曹亮
Owner 江苏中康药物科技有限公司
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