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Method for determining the absolute number density of particles in solution

A particle and number technology, applied in the field of determining the number density of particles in a solution, can solve problems such as time-consuming, low accuracy, and difficulty in determining the absolute number and distribution of particles

Active Publication Date: 2012-02-15
WYATT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Unfortunately, determining absolute particle counts and distributions has been difficult and time-consuming
The need for rapid devices capable of determining these quantities, even with suitably low precision, has long been recognized

Method used

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  • Method for determining the absolute number density of particles in solution
  • Method for determining the absolute number density of particles in solution
  • Method for determining the absolute number density of particles in solution

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Embodiment Construction

[0021] The present invention is readily understood with reference to a monodisperse aqueous suspension of uniform spherical particles. Despite their simplicity, such samples are among the most common and important examples where absolute particle number density needs to be determined. Examples of such samples include well known polystyrene emulsions, or PSL spheres, such as those manufactured by Duke Scientific Corporation of Palo Alto, CA and JSR Corporation of Tokyo, Japan. Such PSL samples are annotated with their average size and mass fraction. Generally, for particles with diameters below 1000 nm, the mass of PSL spheres per milliliter of solution is expressed in %, that is, 1% means 1 gram (gm) of PSL spheres per milliliter (ml) of solution. Therefore, calculation of the actual number density present per milliliter of solution requires prior knowledge of the mean particle size, the PSL mass density, ie, about 1.05, and the percentage of PSL that represents the grams of ...

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Abstract

A method for determining the absolute number density of particles in a solution based on a light scattering method is disclosed. The light scattering photometer is calibrated to measure the Rayleigh ratio at various angles, ie the scattered light in the field of view corresponding to unit incident intensity, unit illuminated volume, and unit steradian opposite the detector. For the calculated numbers to be accurate, the irradiated particles should be effectively monodisperse. An incident beam is impinged on the sample particle, excess Rayleigh ratios are measured at multiple angles, and from these excess Rayleigh ratios an effective size is calculated which in turn is used to calculate the differential scattering intensity at each angle. The number of particles per unit volume unit is then determined by dividing the measured excess Rayleigh ratio by the corresponding differential scattering intensity.

Description

technical field [0001] The present invention relates to a method and apparatus for determining the number of particles, and more particularly to a method and apparatus for determining the number density of particles in solution using light scattering measurements. Background technique [0002] Determining the absolute particle number in a liquid suspension is an important requirement for many types of particle-based applications. In the field of medicine, for example, particles such as liposomes, microcapsules, viruses and emulsions are often used in conjunction with the release of drugs or agents. The therapeutic benefit of these particulate drugs relies on a priori knowledge of the amount of drug being released, which requires accurate measurement of the number of particles per unit volume delivered to the patient. [0003] For viral vectors, such as genetically engineered parvovirus capsids and viruses designed to introduce heterologous genes into target cells, described...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/06G01N21/59G01N15/10G01N15/02G01N21/51
CPCG01N21/51G01N2030/003
Inventor P·J·怀亚特M·J·韦达
Owner WYATT TECH
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