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Method for determining blushred rabdosis A and rosmarinic acid contents in blushred rabdosis and its preparation

A technology of oridonin and rosmarinic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that there is no accurate method for determining oridonin and rosmarinic acid

Inactive Publication Date: 2005-07-27
张海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The contents of oridinin and rosmarinic acid in Oridicea and its preparations are important parameters for determining the quality standards of Oridinus and its preparations, but there is no one that can accurately determine the contents of Oridinus and its preparations. Determination method of the content of iconidin and rosmarinic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A. Preparation of experimental conditions:

[0042] A, prepare mobile phase: concentration is that analytical pure methanol and weight concentration are 0.5% phosphoric acid aqueous solution to be mixed with mobile phase 100ml with the ratio of 1: 0.5;

[0043] b. Chromatographic column requirements: the chromatographic column is a CLC-ODS column, the column height×diameter is 100mm×2mm, the pore diameter of the capillary column is 2μm, and the column temperature is 20°C;

[0044] c. Detection wavelength: 340mm;

[0045] d. Flow rate: 0.5ml / min;

[0046] B. Preparation of reference substance solution:

[0047] Precisely weigh analytically pure oridonin and rosmarinic acid reference substances, respectively add analytically pure methanol to make a solution containing 1 mg of oridonin or rosmarinic acid per 1 ml of solution;

[0048] C, preparation of the test solution:

[0049] Accurately weigh 1g of the powder of Rubescens to be tested, extract twice with a KQ-700E ...

Embodiment 2

[0053] A. Preparation of experimental conditions:

[0054] A, prepare mobile phase: concentration is that analytical pure methanol and weight concentration are 0.5% phosphoric acid aqueous solution to be mixed with mobile phase 100ml with the ratio of 1: 2;

[0055] b. Chromatographic column requirements: the chromatographic column is a CLC-ODS column, the column height × diameter is 200mm × 10mm, the pore diameter of the capillary column is 10μm, and the column temperature is 35°C;

[0056] c. Detection wavelength: 350mm;

[0057] d. Flow rate: 1ml / min;

[0058] B. Preparation of reference substance solution:

[0059] Precisely weigh analytically pure oridonin and rosmarinic acid reference substances, respectively add analytically pure methanol to make a solution containing 1 mg of oridonin or rosmarinic acid per 1 ml of solution;

[0060] C, preparation of the test solution:

[0061] Accurately weigh 1g of the powder of the Cordyceps japonicus tablets to be tested, extra...

Embodiment 3

[0065] A. Preparation of experimental conditions:

[0066] A, prepare mobile phase: concentration is that analytical pure methanol and weight concentration are 0.5% phosphoric acid aqueous solution to be mixed with mobile phase 100ml with the ratio of 1: 1;

[0067] b. Chromatographic column requirements: the chromatographic column is a CLC-ODS column, the column height × diameter is 150mm × 6.0mm, the pore diameter of the capillary column is 5 μm, and the column temperature is 30°C;

[0068] c. Detection wavelength: 335mm;

[0069] d. Flow rate: 0.8ml / min;

[0070] B. Preparation of reference substance solution:

[0071]Precisely weigh analytically pure oridonin and rosmarinic acid reference substances, respectively add analytically pure methanol to make a solution containing 1 mg of oridonin or rosmarinic acid per 1 ml of solution;

[0072] C, preparation of the test solution:

[0073] Accurately weigh 1g of the to-be-tested Radix Grass syrup, extract twice with a KQ-700...

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Abstract

A method using liquid chromatograph to measure content of Rabdosia rubescens A element and rosemary acid in high efficiency includes preparing conditions for test, preparing control sample solution, preparing tested sample solution and carrying out measurement.

Description

technical field [0001] The invention relates to a method for determining the contents of oridonin A and rosmarinic acid in Rubescens orioides and its preparations. Background technique [0002] Donglingcao is a plant of the family Lamiaceae, the genus Camellia. Sweet and bitter in taste, slightly cold in nature, it has the functions of clearing away heat and detoxification, reducing inflammation and relieving pain, invigorating the stomach and activating blood circulation. For the treatment of pyretic carbuncle, blood stasis syndrome and other diseases. [0003] The chemical constituents in Rubescens sativa are very complex, and Rubescensin A and rosmarinic acid are effective active ingredients of Rubescens sativa and its preparation extracts. [0004] Oridonin is an anticancer drug, which has obvious therapeutic effects on various cancers and has few side effects. In vivo experiments proved that Rubescensine A injected intraperitoneally at 10 mg / kg had obvious anti-tumor...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/04G01N30/60
Inventor 张海
Owner 张海
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