Method for preparing vaccine of fowl infectious coryza
A poultry infectious rhinitis and vaccine technology, applied in the field of bioengineering, can solve the problems of low protection rate of virus attack and low bacterial content in culture solution, and achieve the effects of early immunity, long immunity period and small side effects
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Embodiment 1
[0015] Haemophilus paragallinarum standard strain A or C is revived as a bacterial strain, inoculated in a liquid medium and cultured for 36 hours, and formalin solution is added to make the content reach 0.5%. Shake well and inactivate at 37°C for 18 hours. Before the bacteria are inactivated, the total number of bacteria in the culture is determined according to the ministerial standard, and the bacteria content is generally 10 10-11 CFU / ml. Take the inactivated bacteria solution and inoculate it in the chocolate culture medium for 48 hours, and set aside when there is no bacterial growth. Add sterile 4% Tween-80 to the sterilized solution, add sterile levamisole hydrochloride at 5%, add sodium selenite vitamin E injection at 20%, and adjust pH 7.0 to be the water phase; in No. 10 white oil Add 6% Siben-80, and then add 2% aluminum stearate, the oil phase after sterilization. In a high-pressure emulsification pump, the water phase and the oil phase are mixed and emulsifie...
Embodiment 2
[0018] After the standard strains of Haemophilus paragallinarum serotypes A and C are inoculated in the liquid medium and cultured for 36 hours, formalin solution is added to make the content reach 0.5%. Shake well and inactivate at 37°C for 18 hours. Take the inactivated bacteria solution and inoculate it in the chocolate culture medium for 48 hours, and set aside when there is no bacterial growth. Add sterile 4% Tween-80 to the sterilized solution, add sterile levamisole hydrochloride at 5%, add sodium selenite vitamin E injection at 20%, and adjust pH 7.0 to be the water phase; in No. 10 white oil Add 6% Siben-80, and then add 2% aluminum stearate, the oil phase after sterilization. In a high-pressure emulsification pump, the water phase and the oil phase are mixed and emulsified at a ratio of 1:1 to prepare chicken infectious rhinitis vaccine, which contains 100 million bacterial cells per 0.5ml.
[0019] The vaccine is suitable for the immunization of poultry in various...
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