Method for preparing vaccine of fowl infectious coryza

A poultry infectious rhinitis and vaccine technology, applied in the field of bioengineering, can solve the problems of low protection rate of virus attack and low bacterial content in culture solution, and achieve the effects of early immunity, long immunity period and small side effects

Inactive Publication Date: 2006-01-25
SHANGHAI JIAO TONG UNIV
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AI Technical Summary

Problems solved by technology

During the preparation of the vaccine, the bacterial content in the culture solution is low, so that the protection rate after immunization is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Haemophilus paragallinarum standard strain A or C is revived as a bacterial strain, inoculated in a liquid medium and cultured for 36 hours, and formalin solution is added to make the content reach 0.5%. Shake well and inactivate at 37°C for 18 hours. Before the bacteria are inactivated, the total number of bacteria in the culture is determined according to the ministerial standard, and the bacteria content is generally 10 10-11 CFU / ml. Take the inactivated bacteria solution and inoculate it in the chocolate culture medium for 48 hours, and set aside when there is no bacterial growth. Add sterile 4% Tween-80 to the sterilized solution, add sterile levamisole hydrochloride at 5%, add sodium selenite vitamin E injection at 20%, and adjust pH 7.0 to be the water phase; in No. 10 white oil Add 6% Siben-80, and then add 2% aluminum stearate, the oil phase after sterilization. In a high-pressure emulsification pump, the water phase and the oil phase are mixed and emulsifie...

Embodiment 2

[0018] After the standard strains of Haemophilus paragallinarum serotypes A and C are inoculated in the liquid medium and cultured for 36 hours, formalin solution is added to make the content reach 0.5%. Shake well and inactivate at 37°C for 18 hours. Take the inactivated bacteria solution and inoculate it in the chocolate culture medium for 48 hours, and set aside when there is no bacterial growth. Add sterile 4% Tween-80 to the sterilized solution, add sterile levamisole hydrochloride at 5%, add sodium selenite vitamin E injection at 20%, and adjust pH 7.0 to be the water phase; in No. 10 white oil Add 6% Siben-80, and then add 2% aluminum stearate, the oil phase after sterilization. In a high-pressure emulsification pump, the water phase and the oil phase are mixed and emulsified at a ratio of 1:1 to prepare chicken infectious rhinitis vaccine, which contains 100 million bacterial cells per 0.5ml.

[0019] The vaccine is suitable for the immunization of poultry in various...

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Abstract

A process for preparing the vaccine of avian infectious rhinitis includes such steps as reanimating the chicken's parahemophilum CVCC 258 and CVCC 269, continuous passage, inoculating to chicken embryo, separating bacterial seeds, reproducing in liquid culture medium, deactivating, adding the injection containing levamisole hydrochloride, sodium selenite and VE, and preparing oily emulsion. Its advantages are quickly taking its effect (7 days) and long immune time (6 months).

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a preparation method of an avian infectious rhinitis vaccine. Belongs to the field of bioengineering. Background technique [0002] Chicken infectious rhinitis is a respiratory disease of chickens, and it is one of the important infectious diseases that endanger the development of chicken industry at present. The disease is caused by Haemophilus paragallinarum. Haemophilus paragallinarum CVCC258 and CVCC269 are the strains in the "Chinese strain catalog" (English version, published by Machinery Industry Press in October 1992, edited by China Microbial Strain Collection Management Committee), China Veterinary Microbiology Collection Center as the Chinese The organization under the Microbial Cultures Preservation Management Committee guarantees to provide the microorganisms in the "Catalogue of Chinese Cultures" for domestic and foreign teaching, scientific research and product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/102A61P31/04A61P11/00
Inventor 刘永德叶陈梁吴祖立汪毅
Owner SHANGHAI JIAO TONG UNIV
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