Enzymatic method for determining potassium ion content and potassium ion diagnosis kit

A diagnostic kit and potassium ion technology are applied in the field of medical examination and determination, which can solve the problems of complicated operation, insufficient time, and the inability of a biochemical analyzer to simultaneously measure the content of potassium ions and sodium ions, and achieve the effects of high specificity and accurate test results.
CN1786187AInactive Publication Date: 2006-06-14SUZHOU ANJ BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2006-06-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to enzyme method measuring kalium ion content method and its diagnosis kit. It is utilized kalium ion activation pyruvate kinase specificity to couple hexokinase, glucose phosphate dehydrogenase, 6-phosphogluconolactone and phosphogluconate dehydrogenase to react, reduce dichotomy type oxidizing coenzyme to dichotomy reducing type. To measure dominant wavelength 340nm absorbance rising speed can quantificationally reflect sample kalium ion content. The method has high specificity, good accuracy. The diagnosis kit can be made into bi-agent or tri-agent to reduce cross influence. The method can be quickly measured by ultraviolet / visible light analyzer or semi / full automatic biochemical analysis. Its measuring cost is low; and it is convenient for generalization and application.
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Description

technical field

[0001] The invention relates to a method for measuring potassium ion content in samples such as serum and plasma by enzymatic method, and a potassium ion diagnostic kit prepared by applying the method, which belongs to the technical field of medical inspection and determination. Background technique

[0002] The content of potassium ions in serum (referred to as "serum potassium") has very important clinical significance. Under normal circumstances, there is a reasonable reference value range for blood potassium in the human body. Hypokalemia occurs when serum potassium falls below the lower reference limit due to renal tubular disease, hyperaldosteronism, hyperinsulinemia, metabolic alkalosis, or the use of insulin for diabetic ketosis, use of diuretics Potassium ions are transferred to the inside of cells during the treatment of certain diseases; when blood potassium is higher than the upper limit of the reference value, it may be caused by glomerular disea...

Claims

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