Separation and authentication method for denitrifying bacteria with anaerobic ammoxidation activity

A technology of anaerobic ammonium oxidation and denitrifying bacteria, which is applied in anaerobic digestion treatment, biochemical equipment and methods, microbial measurement/inspection, etc. OK, etc.

Inactive Publication Date: 2006-07-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of using Percoll density gradient centrifugation technology is that the isolated bacteria contain a small amount of miscellaneous bacteria. When used as bacteria species for microbiological research, the results obtained are often questioned; the growth conditions of the isolated strains are difficult to determine, and it is not easy to amplify in large quantities.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The steps of the denitrifying bacteria separation method with anammox activity are as follows:

[0038] 1. In this example, the activated sludge in the aeration tank of the urban sewage treatment plant is selected as the separation source of denitrifying bacteria with anammox activity.

[0039] 2. To add NaNO 2 and (NH 4 ) 2 SO 4 The basic inorganic salt medium with a small amount of yeast paste is used as the enrichment substrate, and the anammox flocs are cultivated by continuous culture.

[0040] 3. Take the anammox flocs, and gently break them with ultrasonic waves to prepare a primary bacterial suspension, and then dilute them step by step to prepare a series of bacterial suspensions.

[0041] 4. Using the denitrification medium, use the anaerobic plate streak method to separate the denitrifying bacteria, and anaerobic culture at 28-30 °C. The single colonies grown on the plates were picked into liquid medium and cultured anaerobically at 28-30°C. After cultu...

Embodiment 2

[0055] The steps of the denitrifying bacteria separation method with anammox activity are as follows:

[0056] 1. In this example, waterlogged soil was selected as the isolation source of denitrifying bacteria with anammox activity.

[0057] 2. To add NaNO 2 and (NH 4 ) 2 SO 4 The basic inorganic salt medium with a small amount of yeast paste is used as the enrichment substrate, and the anammox flocs are cultivated by continuous culture.

[0058] 3. Take the anammox flocs, and gently break them with ultrasonic waves to prepare a primary bacterial suspension, and then dilute them step by step to prepare a series of bacterial suspensions.

[0059] 4. Using the denitrification medium, use the anaerobic plate streak method to separate the denitrifying bacteria, and anaerobic culture at 28-30 °C. The single colonies grown on the plates were picked into liquid medium and cultured anaerobically at 28-30°C. After culturing, single colonies were isolated by anaerobic plate streak...

Embodiment 3

[0074] The steps of the denitrifying bacteria separation method with anammox activity are as follows:

[0075] 1. In this example, the activated sludge in the aeration tank of the urban sewage treatment plant is selected as the separation source of denitrifying bacteria with anammox activity.

[0076] 2. To add NaNO 2 and (NH 4 ) 2 SO 4 And the basic inorganic salt medium with a small amount of glucose is used as the enrichment substrate, and the anammox flocs are cultivated by continuous culture.

[0077] 3. Take the anammox flocs, and gently break them with ultrasonic waves to prepare a primary bacterial suspension, and then dilute them step by step to prepare a series of bacterial suspensions.

[0078] 4. Using the denitrification medium, use the anaerobic plate streak method to separate the denitrifying bacteria, and anaerobic culture at 28-30 °C. The single colonies grown on the plates were picked into liquid medium and cultured anaerobically at 28-30°C. After cultu...

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PUM

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Abstract

The invention discloses a separation and authentication method for denitrifying bacteria with anaerobic ammoxidation activity, wherein a novel anaerobic ammonoxidation bacterium segregation and evaluation program is employed, wherein denitrifying bacteria is isolated first, then the capability of ammonia and nitrite simultaneous transformation under anaerobic condition is used as the criterion for confirmation of anaerobic ammonoxidation bacterium, finally the classification status of the bacterium is identified.

Description

technical field [0001] The invention relates to a method for separating and identifying denitrifying bacteria with anammox activity. Background technique [0002] Anaerobic ammonia oxidation (Anaerobic ammonia oxidation, Anammox) was first reported in 1990, it is a biological reaction with ammonia as electron donor and nitrite as electron acceptor, and the reaction product is nitrogen ( <chemistry num="001"> <chem file="200610049500_cml001.xml" / > < / chemistry> ) [1] . In the field of biological denitrification of wastewater, it breaks through the classic nitrification-denitrification theory, and based on this, new biological denitrification processes have been developed, such as short-range nitrification (SHARON)-anammox process [2] , OLAND process [3] and CANON process [4] Wait. The microorganisms involved in these biological denitrification processes are autotrophic bacteria, which do not require additional organic carbon sources, which can not onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C02F3/28
Inventor 郑平胡宝兰
Owner ZHEJIANG UNIV
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