Separation and authentication method for denitrifying bacteria with anaerobic ammoxidation activity

Abstract

The invention discloses a separation and authentication method for denitrifying bacteria with anaerobic ammoxidation activity, wherein a novel anaerobic ammonoxidation bacterium segregation and evaluation program is employed, wherein denitrifying bacteria is isolated first, then the capability of ammonia and nitrite simultaneous transformation under anaerobic condition is used as the criterion for confirmation of anaerobic ammonoxidation bacterium, finally the classification status of the bacterium is identified.

Description

technical field

[0001] The invention relates to a method for separating and identifying denitrifying bacteria with anammox activity. Background technique

[0002] Anaerobic ammonia oxidation (Anaerobic ammonia oxidation, Anammox) was first reported in 1990, it is a biological reaction with ammonia as electron donor and nitrite as electron acceptor, and the reaction product is nitrogen ( <chemistry num="001"> <chem file="200610049500_cml001.xml" / > < / chemistry> ) [1] . In the field of biological denitrification of wastewater, it breaks through the classic nitrification-denitrification theory, and based on this, new biological denitrification processes have been developed, such as short-range nitrification (SHARON)-anammox process [2] , OLAND process [3] and CANON process [4] Wait. The microorganisms involved in these biological denitrification processes are autotrophic bacteria, which do not require additional organic carbon sources, which can not onl...