Cell culturation apparatus

Abstract

This invention discloses a cell cultivatioin device including: a cell cultivation tank with an inlet for seed cells, an inlet for nutrient fluid and a stromal cell adding port on one end, the other end is set with an outlet for cultivated cells, an outlet for metabolized waste solution and a discharge port for stromal cells, at least two supplying tubes for nutrient fluid, one metabolized waste solution reclaim tube and two cell cultivation tubes are set in the cell cultivation tank, other places of which are fully filled with stromal cells, a nutrient fluid circulation device connected with the inlet of the nutrient fluid and the wasted outlet of the cell cultivation tank, a cell providing device connected with the seed cell inlet of the tank and a cell collecting device connected with the outlet of the cultivated cells of the tank.

Description

technical field [0001] The invention relates to a cell culture device, in particular to a device capable of rapidly culturing in large quantities in vitro to obtain specific cells, especially stem cells and their daughter cells of different degrees of differentiation. Background technique [0002] Stem cells are considered ideal seed cells for cell therapy and tissue engineering because of their self-renewal and multi-directional differentiation potential. Both cell therapy and tissue engineering require a considerable number of stem cells. However, the source of stem cells is limited. The current planar culture operations such as ordinary multi-well plates and square flasks are complicated. The intermittent changes in the growth microenvironment have a great impact on cell growth. The number of harvested cells It is far from reaching the clinical needs. At the same time, directly adding a combination of cytokines to the culture system will greatly increase the cost of cult...

AI Technical Summary

Problems solved by technology

[0003] At the same time, in the current methods of inducing stem cells to differentiate into specific cell types (such as nerve cells, muscle cells, insulin-secreting cells, osteoblasts, fat cells, etc.), most of them add exogenous chemical reagents and cytokine combinations to create stem cells. In the microenvironment of directed differentiation, the cost of culture is still high, and at the same time, various factor reagents appear at different time points with the consumption of time, there are concentration gradients, and the induction system is not stable enough.

Images