Tissue culture quick-breeding method for polygonum multiflorum

A technology of tissue culture and Polygonum multiflorum, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of low yield and mixed varieties, and achieve the effects of good growth, saving land occupation, and saving cultivation costs

Inactive Publication Date: 2007-03-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

At present, although there are artificial cultivations in Guangdong, Shandong, Henan and other places, the varieties are mixed and the yield is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take the following steps to propagate Polygonum multiflorum:

[0018] 1. Take the annual branches of Polygonum multiflorum, soak it in washing powder solution and gently rinse the surface with a soft brush for 5 minutes, then rinse it with tap water, then perform aseptic operation in an ultra-clean workbench, and disinfect it with 0.1% mercuric chloride for 4~ After 5 minutes, rinse with sterile water for 5 times, blot the water with sterile filter paper, and finally cut it into 1-2 cm long stems with lateral buds, and inoculate them into the induction medium.

[0019] 2. Primary induction culture. Inoculate the stem section with axillary buds on the induction medium, the composition of the induction medium is: take MS as the basic medium, add 6-BA1.0mg / L+IBA0.1mg / L, the amount of sucrose is 3%, and the agar is 0.7%, pH 5.8. The medium was sterilized at 121°C for 17min. The culture temperature is 25+2°C, fluorescent lamps are used for lighting, the light intensity is...

Embodiment 2

[0025] Except that the concentration and proportioning of the growth regulator used in the rooting medium are 6-BA0.5mg / L+IBA1.0mg / L, all the other are the same as in Example 1.

Embodiment 3

[0027] Except that the concentration and proportion of the growth regulator used in the subculture medium were 6-BA1.5mg / L+IBA0.1mg / L, the rest were the same as in Example 1.

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PUM

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Abstract

The present invention provides a tissue culture fast-propagation method of Chinese medicinal plant polygonum multiflorum. Said method mainly includes the following several steps: preparing explant, induction culture, differentiation culture and rhizogenetic culture. Besides, said invention also provides the concrete requirements of the above-mentioned every step and its concrete operation method.

Description

technical field [0001] The invention relates to a plant regeneration method through tissue culture technology, in particular to a tissue culture rapid propagation method of Polygonum multiflorum. Background technique [0002] Polygonum multiflorum, as a kind of rare and valuable bulk Chinese herbal medicine in our country, is not only used in traditional Chinese medicine, but also can be used to prepare shampoo, Shouwu wine, health care products, food and feed additives, etc. The development prospect is very broad. However, in recent decades, The predatory excavation of wild Polygonum multiflorum is very serious, resulting in a sharp decline in the wild resources of Polygonum multiflorum, causing serious damage to local vegetation and regional ecological balance. Therefore, it is of great ecological and economic significance to protect the existing wild resources of Polygonum multiflorum and vigorously develop artificial cultivation of Polygonum multiflorum. Although there ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 曹福亮汪贵斌
Owner NANJING FORESTRY UNIV
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