Constitution method for silicosis peope serum protein finger print

A serum protein and construction method technology, which is applied in the field of serum protein fingerprint construction of silicosis population, can solve the problem of large index overlap and achieve excellent specificity and sensitivity

Inactive Publication Date: 2007-03-28
MEDICAL COLLEGE OF CHINESE PEOPLES ARMED POLICE FORCE
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Problems solved by technology

Although the results of biomarkers are significant, these indicators overlap between different types of pulmonary interstitial diseases, and they cannot be used as biomarkers for diagnosis and differential diagnosis.

Method used

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  • Constitution method for silicosis peope serum protein finger print
  • Constitution method for silicosis peope serum protein finger print
  • Constitution method for silicosis peope serum protein finger print

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Experimental program
Comparison scheme
Effect test

Embodiment

[0024] 1. Research object and grouping method

[0025] 1 Research object and grouping.

[0026] Strictly follow the national diagnostic criteria for pneumoconiosis (GBZ70-2002) to select silica dust workers (stage 0), suspected silicosis (stage 0+), and silicosis stage I, with 120 people in each group, all male, aged 41.3-66 years , check blood pressure, electrocardiogram, blood routine, liver function and lung function, etc., exclude those suffering from cardiovascular, liver and other organic diseases and other lung diseases, and carefully log in the relevant epidemiological data one by one in the form of questionnaires, Unified high-kV X-ray posteroanterior chest film (120-150KV, 100mA, 0.03-0.05s). Another 120 age-matched healthy males who were not exposed to silica dust were selected as the normal control group.

[0027] 2 Materials and Instruments

[0028] Urea, acetonitrile, trifluoroacetic acid, protease inhibitors, Hepers, sinapic acid (Sinapinic acid, SPA), CHAPS,...

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Abstract

The invention relates to construction of silicosis crowd serum protein fingerprint, the method is: (1) Sampling the early morning fasting venous blood from the control group, the dust group, the suspect silicosis group and silicosis phase I group, separating serum and packing to be frozen; (2)Putting the serum samples of each group into CM10 chips and achieving the enrichment purification of the target protein or polypeptide group and hetero-protein elution; (3) Using protein micro scanner to read chips, access to the experimental group crowd serum protein map; (4) Using bioinformatics software to analyze spectrometry data, picking out difference protein or polypeptide peak, establishing serum protein classification and phased tree diagram of silicosis crowds; (5) Correcting and testing the sensitivity and the specificity of the classification tree diagram by blind way testing the samples of silicosis and the normal population. The crowd silicosis fingerprint can be used to give early discovery, early diagnosis and early treatment.

Description

technical field [0001] The invention relates to the construction of serum protein fingerprints for silicosis populations. Using the fingerprints can achieve high specificity and high sensitivity for the classification of silicosis populations, and provide beneficial help for early detection, early diagnosis and early treatment of silicosis patients. Background technique [0002] Silicosis is a systemic disease mainly caused by pulmonary interstitial fibrosis caused by inhalation of silica dust. The 2004 my country Occupational Disease Statistics Report released by the Ministry of Health shows that the cumulative number of pneumoconiosis cases in the country is 580,000, and the number of suspected pneumoconiosis cases is more than 600,000. Silicosis accounts for nearly 80% of pneumoconiosis cases. The annual direct economic loss caused by pneumoconiosis to the country is about 9 billion yuan, and the indirect economic loss cannot be estimated. Controlling the dust concentra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/72A61B5/157
Inventor 王世鑫赵学峰何冰曾家伟王奕飞
Owner MEDICAL COLLEGE OF CHINESE PEOPLES ARMED POLICE FORCE
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