Application of honokiol in preparing sensitization agent for tumour chemotherapy

A honokiol and sensitizer technology, applied in the field of natural plant monomer compounds, can solve problems such as neutropenia, hypotension, deficiency, etc., and achieve the goal of overcoming tumor drug resistance, low toxicity, and protecting normal human cells Effect

Inactive Publication Date: 2007-05-09
ZHEJIANG UNIV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chemotherapy (chemotherapy) is one of the main treatment methods for cancer. For half a century, tumor chemotherapy has developed rapidly. Currently, chemotherapy alone can cure choriocarcinoma, testicular seminoma, acute lymphoblastic leukemia, etc. , Long-term remission can be obtained for some tumors, such as breast cancer, Wilms tumor, granulosa cell leukemia, etc. However, while improving the cure rate and quality of life of cancer patients, chemotherapy will inevitably produce many adverse reactions, such as kidney disease. Toxicity, cardiotoxicity, bone marrow toxicity, etc.
[0003] Common chemotherapeutic drugs such as doxorubicin and daunorubicin will cause certain cardiotoxicity after being applied to the human body: it can cause sudden tachycardia, acute heart failure, dyspnea, etc., to some extent, anthracene The cardiotoxicity of cyclic antibiotics has become the main factor that limits the dosage of drugs used in chemotherapy; paclitaxel can cause allergic reactions, and reactions such as urticaria, respiratory distress, and hypotension can occur after medication; etoposide can cause severe bone marrow suppression, resulting in granulocytopenia or even deficiency);
[0004] In the clinical chemotherapy of malignant tumor patients, in order to kill tumor cells to the greatest extent and improve the therapeutic effect, the dose of drugs given is generally the "maximum tolerable dose". Therefore, the toxic and side effects of chemotherapy drugs have become It is an important factor that restricts the dosage of chemotherapy drugs and thus restricts the effect of clinical chemotherapy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of honokiol in preparing sensitization agent for tumour chemotherapy
  • Application of honokiol in preparing sensitization agent for tumour chemotherapy
  • Application of honokiol in preparing sensitization agent for tumour chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Honokiol increases the sensitivity of non-drug-resistant tumor cells to chemotherapeutic drugs

[0025] (1) Experimental materials:

[0026] Cell lines: The cells used in this experiment were human promyelocytic leukemia cells HL60 and human breast cancer cells MCF-7 from the American Type Culture Collection (ATCC) company; honokiol, China National Institute of Drugs and Biological Products. Tetramethylazolium blue (MTT) powder and dimethyl sulfoxide (DMSO) were purchased from Sigma, USA; RPMI-1640 culture medium and trypsin were purchased from Promega, USA; 96-well plates were purchased from Falcon, USA.

[0027] Instruments: level shaker; ELISA; cell culture incubator; ultra-clean bench.

[0028] (2) Experimental method

[0029] The above tumor cells were all cultured in RPMI-1640 medium containing 10% calf serum. Cells were inoculated into 96-well cell culture plates, after adding different concentrations of honokiol and chemotherapeutic drugs, the corr...

Embodiment 2

[0040] Example 2: Honokiol overcomes P-gp-mediated drug-resistant tumor cell resistance to certain chemotherapeutic drugs

[0041] (1) Experimental materials:

[0042] Cell line: The P-gp-mediated tumor drug resistance cell-MCF-7ADR used in this experiment comes from the American Type Culture Collection (ATCC) company; honokiol, China National Institute of Drugs and Biological Products. Tetramethylazolium blue (MTT) powder and dimethyl sulfoxide (DMSO) were purchased from Sigma, USA; RPMI-1640 culture medium and trypsin were purchased from Promega, USA; 96-well plates were purchased from Falcon, USA.

[0043] Instruments: level shaker; ELISA; cell culture incubator; ultra-clean bench.

[0044] (2) Experimental method

[0045] MCF-7ADR cells were cultured in RPMI-1640 medium containing 10% calf serum. Cells were inoculated into 96-well cell culture plates, after adding different concentrations of honokiol and chemotherapeutic drugs, the corresponding volume of culture medium...

Embodiment 3

[0055] Example 3: Honokiol overcomes MRP-mediated drug-resistant tumor cell resistance to certain chemotherapeutic drugs

[0056] (1) Experimental materials:

[0057] Cell line: The MRP-mediated tumor drug resistance cell-HL60 ADR used in this experiment comes from the American Type Culture Collection (ATCC) company; honokiol, China National Institute of Drugs and Biological Products. Tetramethylazolium blue (MTT) powder and dimethyl sulfoxide (DMSO) were purchased from Sigma, USA; RPMI-1640 culture medium and trypsin were purchased from Promega, USA; 96-well plates were purchased from Falcon, USA.

[0058] Instruments: level shaker; ELISA; cell culture incubator; ultra-clean bench.

[0059] (3) Experimental method

[0060] HL60 ADR cells were cultured in RPMI-1640 medium containing 10% calf serum. Cells were inoculated into 96-well cell culture plates, after adding different concentrations of honokiol and chemotherapeutic drugs, the corresponding volume of culture medium w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

An application of honokiol (C18H18O2) along with its pharmacologically acceptable excipient or carrier in preparing the sensitizer for the chemicotherapy of cancer is disclosed. Said sensitizer is almost non-toxic to the normal cells of human body.

Description

technical field [0001] The invention belongs to the application of natural plant monomer compounds, and relates to the new application of honokiol extracted from plants, in particular to the application of honokiol in the preparation of common chemotherapy drugs as tumor chemotherapy sensitizers. Background technique [0002] Chemotherapy (chemotherapy) is one of the main treatment methods for cancer. For half a century, tumor chemotherapy has developed rapidly. Currently, chemotherapy alone can cure choriocarcinoma, testicular seminoma, acute lymphoblastic leukemia, etc. , Long-term remission can be obtained for some tumors, such as breast cancer, Wilms tumor, granulosa cell leukemia, etc. However, while improving the cure rate and quality of life of cancer patients, chemotherapy will inevitably produce many adverse reactions, such as kidney disease. Toxicity, cardiotoxicity, bone marrow toxicity, etc. [0003] Common chemotherapeutic drugs such as doxorubicin and daunorub...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61K9/00A61P35/00
Inventor 胡汛徐栋
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap