Method for building poplar embryogenic cell generation technology system

A technology of embryogenic cells and establishment methods, applied in the field of establishment of poplar embryogenic cell generation technology system, can solve the problems of restricting the progress of conventional breeding and long life cycle of forest trees, etc.

Inactive Publication Date: 2007-05-16
上海光兆植物速生技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the long life cycle of forest trees limit

Method used

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Embodiment 1

[0017] A method for establishing a poplar embryogenic cell generation technology system, that is, reaching the most period of poplar embryogenic cell generation by tissue culture technology, comprising the following steps:

[0018] The first step: select 40 days of tissue culture age and 4-5cm bottle seedlings of European and American poplar (black poplar) tissue culture seedlings, and cut the stem section into 1mm as explants;

[0019] Step 2: Dedifferentiate on XF medium, MS+2, 4D 0.2mg / L+NAA 0.1mg / L+sucrose 30g / L+agar 4gg / L for 120 hours;

[0020] The third step: the callus obtained in the second step is transferred to the Y-2 medium, that is, MS+6-BA0.2mg / L+KT 0.2mg / L+IAA 0.05+sucrose 30g / L+agar 4g / L , carry out redifferentiation for 72 hours, at this time is the period when embryogenic cells occur most.

Embodiment 2

[0022] A method for establishing a poplar embryogenic cell generation technology system, that is, reaching the most period of poplar embryogenic cell generation by tissue culture technology, comprising the following steps:

[0023] The first step: select Populus tomentosa tissue culture seedlings with a seedling age of 30 days and a seedling height of 4 to 5 cm, and cut the stem segments into 1.5 mm as explants;

[0024] Step 2: Inoculate on XF medium, namely MS+NAA 0.1mg / L+ZT 0.05mg / L+sucrose 30g / L+agar 4g / L for dedifferentiation for 140 hours;

[0025] The third step: the callus obtained in the second step is transferred to the Y-2 medium, that is, MS+KT 0.2mg / L+ZT 0.1mg / L+IAA 0.1mg / L+sucrose 30g / L+agar 4g / L , Carry out redifferentiation for 96 hours, which is the period when embryogenic cells occur most.

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Abstract

The invention discloses a building method of poplar embryonic cell generating technical system, which comprises the following steps: 1. cutting nursery-grown plant with seedling age at 30-50d and seedling height at 4-5cm into 1-2mm as explant; 2. seeding on the XF culture medium to removing differentiation for 100-140h; 3. switching the callus on the Y-2 culture medium; differentiating 72-96h. The invention shortens wood living period, which cultures poplar embryonic cell with totipotence to help poplar breed.

Description

technical field [0001] The method for establishing the poplar embryogenic cell generation technology system of the invention relates to a method for obtaining a large amount of poplar embryogenic cells by selecting appropriate explants and using a reasonable hormone ratio and cultivation method. Background technique [0002] Embryogenic cells (embryogenic cells) are similar to meristematic cells, usually small, nearly round, with large nuclei and nucleoli, highly stained, dense cytoplasm, and can directly develop into somatic embryos. cell. [0003] Embryogenesis is one of the important ways for the formation of pluripotent expression of plant cells in cell engineering. is of great significance. With the development of cell biology and molecular biology and other theories and technologies, embryogenic cell generation technology will play a greater role in plant disease resistance, insect resistance, stress resistance, improved traits, and genetic engineering breeding. [...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 苏敏宋学孟董举文张艳庞瑞华许雄山章振华王凌健张承妹
Owner 上海光兆植物速生技术有限公司
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