Preparation process of autogenous high-immunity serum for treating post-weaning multi systemic wasting syndrome of baby pig

A technology of piglet weaning and hyperimmune serum, which is applied in the direction of antiviral agents and antibodies, can solve the problems of low titer of healthy serum antibodies, inability to meet the needs of prevention and treatment, and single pathogens of disease materials, so as to save the cost of treatment drugs and significantly Economic benefit, the effect of high pathogenic content

Inactive Publication Date: 2007-05-30
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The healthy serum prepared by the prior art is collected from pigs in a state of natural infection, and most of them are collected when the pathog

Method used

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Embodiment 1

[0021] Suspected piglet weaning multisystemic wasting syndrome (PMWS) has been prevalent in an intensive 10,000-head pig farm in a certain place for several years, with an incidence rate of 30%-50%, resulting in stunted growth of piglets, increased deaths year by year, and heavy losses. The present invention selects this field as the project implementation base, and at first carries out PCV2 antibody detection to a PMWS suspected case in a pig farm: the PCV2 virus strain cultivated by PK-15 cells is used as the coated antigen after density gradient centrifugation and purification; Content-coated 96-well ELISA plate, 37°C, 2h, washed with PBS (phosphate buffer saline); take the serum of sick pigs with suspected symptoms of PMWS as the sample to be tested; dilute the serum to be tested in multiples, add ELISA coating respectively Antigen-antibody reaction was carried out on the plate, washed with PBS at 37°C for 2 hours; HRP-labeled goat anti-pig IgG at the working concentration ...

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Abstract

The invention discloses a house hyper-immune serum making method to treat multi-system weaning exhaustion syndrome PMWS of pigling, which is characterized by the following: adopting multiple PCR technique to detect main pathogen PCV2 in the organ and the content of synergic infection PPV and PRRSV virus; selecting relative high-virus content organ as raw material to make house organ vaccine; proceeding multi-point injection strengthening immune through inactivated organ vaccine; detecting serum antibody level of immune pig; separating serum through high-antibody level pig; detecting the serum after biological safety as treating hyper-immune serum.

Description

technical field [0001] The invention relates to a method for preparing self-made hyperimmune serum for treating piglet weaning multisystemic wasting syndrome. The prepared hyperimmune serum is used for the prevention and treatment of piglet weaning multisystemic wasting syndrome (PMWS) in intensive pig farms, so as to ensure The safety of veterinary public health in pig farms can reduce the incidence and mortality of PMWS in pig farms. Background technique [0002] Post-weaning multisystemic wasting syndrome (Post-Weaning multi systemic Wasting Syndrome, PMWS) is a new disease of piglets weaning and growing stage caused by porcine circovirus type 2 (Porcinecircovims 2, PCV2), which can lead to weaning piglets and very high mortality rates in finishing pigs. The disease is distributed worldwide and seriously endangers the healthy development of the pig industry, and has received more and more attention from related fields. In addition, because PCV2 is widely present in pigs...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P31/12
Inventor 朱建国华修国张艳玲
Owner SHANGHAI JIAO TONG UNIV
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