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Ascorbic acid production from yeast

Inactive Publication Date: 2005-01-27
UNIV DEGLI STUDI DI MILANO BICOCCA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A deficiency of L-ascorbic acid can impair the production of collagen and lead to joint pain, anemia, nervousness and retarded growth.
If left untreated, scurvy is fatal.
Therefore, it cannot be stored in the body.
However, L-ascorbate transporters have not been described among the yeast genera.
Nevertheless, while Candida cells growing in media containing L-galactono-1,4-lactone accumulate L-ascorbic acid in the medium, accumulation in the medium of L-ascorbic acid from wild-type S. cerevisiae cells has, surprisingly, never been described.
However, characterization of the enzyme catalyzing this step has not been presented in detail.

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  • Ascorbic acid production from yeast
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  • Ascorbic acid production from yeast

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examples

[0110] The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

[0111] Materials and Methods

[0112] 1. Determination of Ascorbic Acid

[0113] Ascorbic acid was determined spectrophotometrically following a method after Sullivan et al. (1955, Assoc. Off. Agr. Chem., 38, 2, 514-518). 135 μl of sample were mixed in a cuvette with 40 μl of H3PO4 (85%). Then 675 μl α,α′-Bipyridyl (0.5%) and 135 μl FeCl3 (1...

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Abstract

Herein is disclosed a method of generating ascorbic acid from yeast. In one embodiment, the yeast is a Zygosaccharomyces spp. or a Kluyveromyces spp. growing in a medium comprising an ascorbic acid precursor. In a second embodiment the yeast is a recombinant yeast growing in a medium comprising an ascorbic acid precursor. Preferably the recombinant yeast is transformed with a coding region encoding an enzyme selected from L-galactose dehydrogenase (LGDH), L-galactono-1,4-lactone dehydrogenase (AGD), D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO) or L-gulono-1,4-lactone oxidase (RGLO). The ascorbic acid precursor is preferably D-glucose, L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid is accumulated in the medium at levels greater than background. Preferably, the yield of the conversion of the precursor to ascorbic acid is preferably at least about 35%.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The present invention relates generally to the field of ascorbic acid production. More particularly, it relates to a process for the production of L-ascorbic acid from yeast, including recombinant yeast. [0003] 2. Description of Related Art [0004] L-ascorbic acid (Vitamin C) is a powerful water-soluble antioxidant that is vital for growth and maintenance of all tissue types in humans. One important role of ascorbic acid is its involvement in the production of collagen, an essential cellular component for connective tissues, muscles, tendons, bones, teeth and skin. Collagen is also required for the repair of blood vessels, bruises, and broken bones. Ascorbic acid helps regulate blood pressure, contributes to reduced cholesterol levels, and aids in the removal of cholesterol deposits from arterial walls. Ascorbic acid also aids in the metabolization of folic acid, regulates the uptake of iron, and is required for the ...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N1/19C12N9/02C12N9/04C12N9/18C12N15/52C12N15/53C12N15/55C12P17/04C12R1/645
CPCC12N9/0006C12N9/001C12P17/04C12N15/52C12N9/18
Inventor PORRO, DANILOSAUER, MICHAEL
Owner UNIV DEGLI STUDI DI MILANO BICOCCA
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