Ascorbic acid production from yeast
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[0110] The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.
[0111] Materials and Methods
[0112] 1. Determination of Ascorbic Acid
[0113] Ascorbic acid was determined spectrophotometrically following a method after Sullivan et al. (1955, Assoc. Off. Agr. Chem., 38, 2, 514-518). 135 μl of sample were mixed in a cuvette with 40 μl of H3PO4 (85%). Then 675 μl α,α′-Bipyridyl (0.5%) and 135 μl FeCl3 (1...
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