Method of treating neutrophil-related diseases with topical anesthetics
a topical anesthetic and neutrophil technology, applied in the field of topical anesthetics for treating neutrophil-related diseases, can solve the problems of emphysematous changes, glucocorticoids have no effect on the long-term decline of pulmonary function, and difficult definition of morphological description
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example 1
Inhibition of Neutrophil Superoxide Production by Lidocaine
[0043] Lidocaine has an inhibitory effect on neutrophil superoxide production. Neutrophils were purified from heparinized venous blood of normal volunteers by sedimentation through a cushion of 1.085 gm / ml Percoll made in PIPES buffer, pH 7.4, supplemented with 50 mM NaCl, 5 mM KCl, 25 mM NaOH, and 5.4 mM glucose) and centrifuged at 2,000 rmp in a Beckman CS-6KR centrifuge for 30 minutes with no brake. Plasma, mononuclear cells, and Percoll layers were removed and the erythrocytes were lysed by osmotic shock. The remaining pellet, which was ˜90% neutrophils, was used in the experiment.
[0044] The superoxide production was measured using cytochrome c reduction in a plate assay at a concentration of 50,000 neutrophils / well and was done in duplicate. There was no superoxide release in the absence of stimulus; TNF-alpha induced superoxide release was almost totally inhibited by 3 mM lidocaine, but PMA induced superoxide product...
example 2
Case Report of Patient with Bronchitis Treated with Lidocaine Inhalation
[0045] The patient was a 36-year-old female first seen in 1996 with a six month history of harsh cough affecting her both during the night and the day. Copious (about 50 ml per day), thick, yellow to white sputum was produced. She was often awakened at night with chest heaviness and dyspnea. Albuterol treatment reduced the cough and minimally alleviated the chest tightness. The patient was treated with prednisone and antibiotics on two occasions and this treatment improved her symptoms, but did not eliminate the sputum production. The patient worked as a scrub nurse in an operating room, but she did not note any worsening of her symptoms while at work or at home. When initially evaluated, the patient was receiving prednisone, 30 mg every other day, Salmeterol twice daily and Asthmacort four inhalations four times a day. Two sputum examinations revealed a striking infiltration with neutrophilic leukocytes.
[0046...
example 3
Treatment of Airway Neutrophilia in Chronic Bronchitis and COPD
[0050] In preliminary studies, it was discovered that lidocaine has potent anti-inflammatory properties with the ability, in vitro, to inhibit cytokine-stimulated eosinophilopoesis and prolongation of survival, activation, respiratory burst, and degranulation of eosinophils. Okada. S. et al., J. Immunol. 160:4010-4017 (1998). Subsequent in vivo studies have shown that lidocaine inhalation reduces or eliminates systemic glucocorticoids in most adult (Hunt, L. W. et al., Mayo Clinic Proc. 71:361-368 (1996)) and pediatric (Decco, M. L. et al., Ann. Allergy Asthma Immunol. 82:29-32 (1999)) patients with steroid-dependent asthma, and is able to replace topical glucocorticoids in patients with mild to moderate asthma. In the latter study, nebulized lidocaine also prevented the rise in peripheral blood eosinophils when topical glucocorticoids were withdrawn.
[0051] In vitro studies show that lidocaine inhibits depolarization o...
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