Method of reducing the harmful effects of orally or transdermally delivered nicotine

Inactive Publication Date: 2006-07-20
VECTOR TOBACCO LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0027] As described above, some of the tobaccos described herein contain an amount of exogenous nicotine. More specifically, some of the tobaccos described herein comprise an amount of exogenous nicotine (e.g., nicotine prepared by extraction of conventional tobacco or synthetically prepared nicotine) so as to adjust the content of the nicotine in the tobacco to a desirable level, which allows for fine adjustments in the amount of nicotine present in a tobacco product that comprises

Problems solved by technology

Although these conventional products of NRT may help tobacco users by suppressing the symptoms of nicotine withdrawal, they do little to satisfy a tobacco user's cravings for the habitual use of the delivery system.
In addition to the fact that conventional NRT does little to quell the secondary factors of addiction, NRT has had only limited success in enabling people to quit tobacco use.
One study, however, goes so far as to sa

Method used

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  • Method of reducing the harmful effects of orally or transdermally delivered nicotine
  • Method of reducing the harmful effects of orally or transdermally delivered nicotine
  • Method of reducing the harmful effects of orally or transdermally delivered nicotine

Examples

Experimental program
Comparison scheme
Effect test

Example

EXAMPLE 1

Isolation and Sequencing

[0301] TobRD2 cDNA (Conkling et. al., Plant Phys. 93, 1203 (1990)) encodes QPTase, which is predicted to be a cytosolic protein. Comparisons of the NtQPT1 amino acid sequence with the GenBank database revealed limited sequence similarity to certain bacterial and other proteins; quinolate phosphoribosyl transferase (QPTase) activity has been demonstrated for the S. typhimurium, E. coli and N. tabacum genes. The NtQPT1 encoded QPTase has similarity to the deduced peptide fragment encoded by an Arabidopsis EST (expression sequence tag) sequence (Genbank Accession number F20096), which may represent part of an Arabidopsis QPTase gene.

Example

EXAMPLE 2

Transformation of Tobacco Plants

[0302] DNA of the QPTase gene, in antisense orientation, is operably linked to a plant promoter (CaMV 35S or TobRD2 root-cortex specific promoter) to produce two different DNA cassettes: CaMV35S promoter / antisense QPTase-encoding gene and TobRD2 promoter / antisense QPTase-encoding gene.

[0303] A wild-type tobacco line and a low-nicotine tobacco line are selected for transformation, e.g., wild-type Burley 21 tobacco (Nic1+ / Nic2+) and homozygous Nic1- / Nic2-Burley 21. A plurality of tobacco plant cells from each line are transformed using each of the DNA cassettes. Transformation is conducted using an Agrobacterium vector, e.g., an Agrobacterium-binary vector carrying Ti-border sequences and the nptII gene (conferring resistance to kanamycin and under the control of the nos promoter (nptII)).

[0304] Transformed cells are selected and regenerated into transgenic tobacco plants called Ro. The Ro plants are grown to maturity and tested for levels ...

Example

EXAMPLE 3

Tobacco Having Reduced Nicotine and / or TSNA Levels

[0306] Tobacco of the variety Burley 21 LA was transformed with the binary Agrobacterium vector pYTY32 to produce a low nicotine tobacco variety, Vector 21-41. The binary vector pYTY32 carried the 2.0 kb NtQPT1 root-cortex-specific promoter driving antisense expression of the NtQPT1 cDNA and the nopaline synthase (nos) 3′ termination sequences from Agrobacterium tumefaciens T-DNA. The selectable marker for this construct was neomycin phosphotransferase (nptII) from E. coli Tn5 which confers resistance to kanamycin, and the expression nptII was directed by the nos promoter from Agrobacterium tumefaciens T-DNA. Transformed cells, tissues, and seedlings were selected by their ability to grow on Murashige-Skoog (MS) medium containing 300 μg / ml kanamycin. Burley 21 LA is a variety of Burley 21 with substantially reduced levels of nicotine as compared with Burley 21 (i.e., Burley 21 LA has 8% the nicotine levels of Burley 21, se...

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Abstract

The present invention generally relates to the reduction of the harmful effects of orally or transdermally delivered nicotine in conventional tobacco-use cessation programs. More specifically, embodiments concern methods of reducing the harmful effects of nicotine intake, associated with conventional tobacco-use cessation programs, by providing tobacco products, which contain a reduced amount of nicotine and/or tobacco specific nitrosamines (TSNAs).

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation-in-part of, and claims the benefit of priority to, international patent application number PCT / US2004 / 016958, filed May 27, 2004, which designated the United States of America and was published in English and which claims the benefit of priority to U.S. provisional patent application No. 60 / 475,945, filed Jun. 4, 2003; this application is a continuation-in-part of and claims the benefit of priority to PCT / US2005 / 10733, filed Mar. 29, 2005, which designated the United States of America and was published in English and which claims the benefit of priority to No. 60 / 557,929, filed Mar. 30, 2004; this application is a continuation-in-part of and claims the benefit of priority to U.S. patent application Ser. No. 11 / 077,752, filed Mar. 10, 2005, which is a continuation of U.S. patent application Ser. No. 10 / 729,121, filed Dec. 5, 2003, now U.S. Pat. No. 6,907,887, which is a continuation of PCT / US2002 / 18040,...

Claims

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Application Information

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IPC IPC(8): A24F47/00
CPCA24B15/20A24B15/243A24B15/245A61K9/007C12N15/8243
Inventor ALBINO, ANTHONYCONKLING, MARKJIN, WENDY
Owner VECTOR TOBACCO LLC
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