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Hyaluronic Acid Derivative and Neural Stem Cells for SCI and PNT Regeneration

a technology of neural stem cells and hyaluronic acid, which is applied in the direction of biocide, prosthesis, drug composition, etc., can solve the problem of less intensive post-operative animal car

Inactive Publication Date: 2009-08-13
BHATIA RAJ KUMAR +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the unambiguous demonstration of regenerating axons in a contusion model poses a new challenge, specifically the delineation of spared and regenerated axons.
Unilateral spinal cord sparing is usually sufficient to maintain bladder and bowel function, which results in less-intensive post-operative animal care.

Method used

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  • Hyaluronic Acid Derivative and Neural Stem Cells for SCI and PNT Regeneration
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  • Hyaluronic Acid Derivative and Neural Stem Cells for SCI and PNT Regeneration

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Embodiment Construction

[0018]Hyaluronic-Acid-Derived Membranes and Fibers: a Multi-Component Scaffold

[0019]The first step of the experimentation of the invention was a broad, in vitro comparison between different versions of hyaluronic acid, partially and totally esterified, N-sulphated, partially amidated (gel), percarboxylated and some auto-crosslinked versions (gel). Particularly suitable for the invention herein described have proved to be the HA esters, and especially so the benzyl ester (HYAFF®11).

[0020]HA at different percentages of esterification (>70%, preferably from 75 to 100%, even more preferably 100% esterification degree) was provided in solid and partially flexible structures: ranging from non-woven meshes and parallel non-woven fibers to woven tubular structures and laminas already available from other studies and tissue engineering applications as skin, vascular tissue, cartilage regeneration. All of the in vitro screening experiments were done following the protocols established for har...

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Abstract

A biomaterial for the treatment of spinal cord or of peripheral nerve injury injury, obtainable by: a) treating a hyaluronic acid derivative with a coating solution promoting Neuronal Stem Cells adhesion, branching and differentiation; b) contacting isolated Neuronal Stem Cells with the hyaluronic acid derivative obtained from step a) and culturing and expanding the absorbed cells in the presence of growth or neurotrophic factors selected from βFGF (basic fibroblast growth factor), CNTF (ciliary neurotrophic factor), BDNF (brain derived neurotrophic factor) and GDNF (glial derived neurotrophic factor) or mixtures thereof.

Description

SUBJECT OF THE INVENTION[0001]The present invention is directed to regeneration of Spinal Cord and Peripheral Nerve Injury after implantation of a scaffold made of a Hyaluronic acid derivative alone or in which Neural Stem cells are seeded.BACKGROUND OF THE INVENTION[0002]Hyaluronic acid is a major component of the extracellular matrix that surrounds migrating and proliferating cells. It is an extremely long, negatively charged polysaccharide, each molecule of which consists of as many as 50,000 replications of the simple disaccharide composed by Glucuronic acid and N-Acetylglucosamine. Indeed, it is important to mention successful in vivo applications of its derivatives in wound healing, diabetic ulcers, vitiligo treatments, laparoscopic adhesion prevention, anti-adhesive surface coatings, cartilage regeneration.[0003]One class of HA derivatives are called auto-crosslinked polysaccharides. In this case the stability if the polymer was obtained by creating cross-linking bonds, but n...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/12C12N5/07C12N5/074C12N5/0797
CPCA61L27/20A61L27/383A61L27/3878A61L27/3895C08L5/08A61P25/00A61P25/02A61P43/00A61F2/02
Inventor BHATIA, RAJ KUMARBHATIA, VIPEN KUMARVISHNOI, ANAND SHANKER
Owner BHATIA RAJ KUMAR
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