Melaleuca quinquenervia extracts and uses of the same

a technology of melaleuca quinquenervia and extract, which is applied in the direction of plant/algae/fungi/lichens, biocide, drug composition, etc., can solve the problems of severe abnormal physiological functions that are difficult to cure, patient worsening, and surgical excision of tumors cannot effectively cure tumors

Inactive Publication Date: 2015-07-30
TAIWAN SUGAR CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The proliferation and metastasis of cancer cells may lead to severe abnormal physiological functions that are difficult to be cured.
However, surgical excision of tumors cannot effectively cure tumors, because incompletely excised tumor cells may continue growing and make the condition of the patient worse.
However, most medicaments used in chemical treatment also have harmful effects on normal cells.
For example, chemical treatment usually induces severe side-effects in cancer patients, which include emesis, baldness, fatigue, hemorrhage, anemia, etc.
However, high energy radiation will simultaneously irradiate normal cells when being used to destroy tumor cells, and such irradiation will result in side-effects, such as leukopenia, fatigue, insomnia, pain, inappetence, etc.
In addition, the therapeutical effect of radiation treatment is poor for some patients in the late stage of the disease.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Melaleuca quinquenervia Extract

[0041]Leaves and branches with leaves of Melaleuca quinquenervia were air dried and smashed by a comminutor, and then processed by the following steps:[0042]i). soaking and extracting 2250 g of smashed Melaleuca quinquenervia with methanol (100%) at room temperature for 2 days, and repeated 3 times to obtain a methanol extract;[0043]ii). filtrating the methanol extract from step i) and concentrating under a vacuum to obtain a crude extract;[0044]iii). adding 1500 ml of pure water to the crude extract of step ii) and mixing the mixture uniformly by ultrasonic oscillation to obtain a methanol extraction fluid;[0045]iv). partitioning the methanol extraction fluid of step iii) by adding 2000 ml of n-hexane thereinto, and then removing the n-hexane phase;[0046]v). partitioning the extraction fluid of step iv) by adding 1800 ml of ethyl acetate thereinto, and then removing the ethyl acetate phase;[0047]vi). partitioning the extraction fluid of...

example 2

Analysis of the Activity of Melaleuca quinquenervia Extract on Inhibiting Wnt / β-Catenin Signal Transduction Pathway

[0049]An analysis system for analyzing the activity of Wnt / β-catenin signal transduction pathway was used in this example as a screening platform (provided from Food Industry Research and Development Institute, Taiwan) for components with anti-tumor activity. The screening platform comprises a human cell line (referred to as “293-bc-GFP cell line”) which can express a fusion protein of β-catenin and green fluorescent protein (GFP) as a reporter gene (referred to as “β-catenin-GFP-fusion protein”). The preparation method of the human cell line for expressing β-catenin-GFP-fusion protein has been described in detail by references (see The development of a functional assay system of Wnt / β-catenin signal pathway, Journal of Chongqing Medical University. 29: 413-416; 2004, which is entirely incorporated hereinto by reference). In addition, a luciferase (Luc) could be used as...

example 3

Cytotoxicity Test of Melaleuca quinquenervia Extract

[0053]3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (referred to as “MTT”) was used to test the survival rate of the 293-bc-Luc cell line treated with the Melaleuca quinquenervia extract prepared from Example 1. First, 293-bc-Luc cell line was cultured with 0.1 ml DMEM culture medium (containing 10% fetal bovine serum, 100 unit / ml penicillin and 100 μg / ml streptomycin) at an initial cell number of 5×104 cells per well in 96-well culture plate in an incubator with 37° C., 5% CO2 overnight. After the cells attached to the culture plate, the original culture medium was removed and fresh 0.1 ml of DMEM medium and 25 μg / ml Melaleuca quinquenervia extract prepared from Example 1 were added to each well of the culture plate and cultured in an incubator with 37° C., 5% CO2 for 24 hours. Thereafter, the culture medium was removed. 20 ml of MTT solution (5 mg / ml) was added to each well of the culture plate and placed in an in...

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PUM

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Abstract

A Melaleuca quinquenervia extract and its uses are provided. The extract is extracted by a first polar solvent, wherein the first polar solvent is a C3-C6 ethanol. The extract is especially useful for inhibiting the Wnt / β-catenin signaling pathway.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to Taiwan Patent Application No. 103102902 filed on Jan. 27, 2014, in the Taiwan Intellectual Property Office, the disclosure of which is incorporated herein in its entirety by reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates to a Melaleuca quinquenervia extract and uses of the same. The extract is useful for inhibiting Wnt / β-catenin signal transduction pathway, and particularly useful for inhibiting the transcription activity of β-catenin and / or inhibiting the combination of β-catenin and T cell factor / lymphoid enhancer factor (TCF / LEF).[0004]2. Description of the Related Art[0005]A tumor is referred to as abnormal lesions of cells. Such lesions are caused by a variety of carcinogenic factors that affect the normal regulation of cells in a local tissue of human body at the gene level and result in abnormal proliferation and lumping of cells, called a ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/61
CPCA61K36/61A61K2236/00A61P35/00
Inventor CHENG, KUN-CHINGCHANG, CHUN-CHINLIOU, JIA-JER
Owner TAIWAN SUGAR CORP
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