Methods for simultaneous amplification of target loci

US20190010543A1Inactive Publication Date: 2019-01-10NATERA
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
NATERA
Publication Date
2019-01-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. Utility application Ser. No. 14 / 918,544, filed Oct. 20, 2015.

[0002] U.S. Utility application Ser. No. 14 / 918,544, filed Oct. 20, 2015, is a continuation-in-part application of U.S. Utility application Ser. No. 14 / 877,925, filed Oct. 7, 2015; a continuation-in-part application of U.S. Utility application Ser. No. 14 / 692,703, filed Apr. 21, 2015; a continuation-in-part application of U.S. Utility application Ser. No. 14 / 538,982 (now U.S. Pat. No. 9,677,118), filed Nov. 24, 2014; and claims the benefit of and priority to U.S. Provisional Application Ser. No. 62 / 148,173, filed Apr. 15, 2015; U.S. Provisional Application Ser. No. 62 / 147,377, filed Apr. 14, 2015; U.S. Provisional Application Ser. No. 62 / 146,188, filed Apr. 10, 2015; and U.S. Provisional Application Ser. No. 62 / 066,514, filed Oct. 21, 2014.

[0003] U.S. Utility application Ser. No. 14 / 877,925, filed Oct. 7, 2015, is a continuation-in-part o...

Claims

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