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Novel lactococcus lactis subspecies lactis isolate wflu-12 and use thereof

Inactive Publication Date: 2020-07-30
PUKYONG NAT UNIV IND ACADEMIC COOPERATION FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a new probiotic isolate, designated Lactococcus lactis subspecies lactis, which has excellent antibacterial activity against pathogens and promotes the growth of fish body. This isolate can be used in a probiotic composition to enhance the immune response of fish and promote their growth. The invention also provides a method of promoting the growth of farmed fish and preventing infection by pathogens. The use of probiotics as a substitute for antibiotics and chemotherapeutics is recommended to avoid the creation of antibiotic-resistant bacteria in the aquatic environment.

Problems solved by technology

However, the use of antibiotics and chemotherapeutics may cause selective pressure, thereby creating antibiotic resistant bacteria in the aquatic environment, increasing antibiotic tolerance in fish pathogens, and subsequently delivering tolerance determinants which exist in bacteria and fish pathogens to the pathogens of land animals and humans.
In Korea, the rapid expansion of the aquaculture industry has been severely impacted by bacterial pathogens.

Method used

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  • Novel lactococcus lactis subspecies lactis isolate wflu-12 and use thereof
  • Novel lactococcus lactis subspecies lactis isolate wflu-12 and use thereof
  • Novel lactococcus lactis subspecies lactis isolate wflu-12 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 2

rial Activity Test

[0059]The antibacterial activity against fish pathogens was tested using the cross-streak method, and the results are shown in Table 2 and FIG. 3. In FIG. 3, the antibacterial activity was confirmed in A. salmonicida (0.4 cm), S. iniae (1 cm), S. parauberis (1.1 cm), V ichthyoenteri (0.4 cm).

TABLE 2Antimicrobial activityaSource ofAeromonasEdwardsiellaPhotobacteriumStreptococcusStreptococcusVibrioVibrioSpeciesisolationsalmonicidatardadamselaeiniaeparauberisanguillarumichthyoenteriIsolates obtainedfrom fish gut*Lc. lactisOlive +++−++++++++WFLUJ2flounderReference strains*Lc. lactisEarthworm +++−−−++KCTC 3899Intestine*Lc. lactisMilk+−−−−−−ATCC 19435*Lc. lactisLeaf +−−−−−−ATCC 29071hopper*Lc. lactisRaw milk−−−−−−−ATCC 19257KCTC: Korean Collection for Type Culture;ATCC: American Type Culture Collection;*Inhibition zone (mm): ++++ = 16-20 mm; +++ = 11-15 mm; ++ = 6-10 mm; + = 1-5 mm; − = no inhibition*The results were recorded using both cross-streaking method and agar we...

example 3

Exclusion Activity

[0063]The following test was carried out having a flounder fed with a general compound feed as a control group and a flounder fed with a feed to which WFLU-12 probiotic was added as an experimental group (probiotic).

[0064]After orally infected with Streptococcus parauberis, the numbers of bacteria in the intestinal and renal tissues were compared (FIG. 5). The average value of Streptococcus parauberis in the intestine and kidney was significantly lower in both week 2 and week 4 of the experimental group as compared with the control group. Therefore, it can be confirmed that the isolate WFLU-12 of the present invention has a function of inhibiting the invasion of streptococcal pathogens. In FIG. 5, the arrows show a significant exclusion of pathogen colonization and invasion in the fish of the experimental group. ((*) and (**) indicate the statistical significance as compared with the control group, which are p<0.05 and p<0.01, respectively. The same applies to all ...

example 4

in Innate Immune Response

[0066]FIG. 6 shows the result of measuring phagocytotic activity and respiratory explosive activity by separating the head from the body in the pilot-scale prey test. The experimental group shows a significantly higher activity at week 2 and week 4, respectively. In FIG. 6, each bar represents an average value for 10 fish and shows a standard error.

[0067]FIG. 7 shows the result of comparing the pro-inflammatory cytokine expression in the experimental group and the control group. From the top, the expression of IL-6 and IL-1β in the intestine, and the expression of IL-6, g-lysozyme, IL-8 and IFN-γ in the kidney were compared, and as a result, it was confirmed that the isolate WFLU-12 induced an increase in the pro-inflammatory cytokine expression and induced an increase in IL-6, IL-8, INF-γ and g-lysozyme in the kidney and an increase in IL-6 in the intestine. Each bar represents an average value for 10 fish and shows a standard error.

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Abstract

The present invention provides a Lactococcus lactis subspecies lactis isolate WFLU-12 with the accession number of KCTC 13180BP, and a use thereof.

Description

TECHNICAL FIELD[0001]The present invention relates to a novel Lactococcus lactis subspecies lactis isolate WFLU-12, and a use thereof.BACKGROUND ART[0002]The world fish food aquaculture production in 2012 was recorded as 44.2 million tons of fish (66%), 15.2 million tons of mollusks (23%), 6.4 million tons of crustaceans (10%), and 0.9 million tons of other species (1%). The inland aquaculture production was 38.6 million tons, which accounts for 58% of the world total aquaculture production in 2012. The proportion of aquaculture in the total fish production is steadily on an increase around the world. In Asia, the fish production of aquaculture exceeded the fish production of the general fishing industry (capture) since 2008, and the fish production of aquaculture accounted for 54% of the total fish production in 2012. This is a remarkably high figure compared to 18% in Europe and less than 15% in other countries, and aquaculture accounts for a very high share in the food industry o...

Claims

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Application Information

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IPC IPC(8): C12R1/46C12N1/20A61K35/744A23K50/80A23K10/18A23K20/195
CPCA23K10/18C12R1/46A23K50/80A61K35/744C12N1/20A23Y2240/41A23K20/195C12R2001/01C12N1/205A61K2039/106A61K39/09A61K39/092A23V2400/231A61K39/025
Inventor KIM, DO-HYUNGNGUYEN, THANH LUANKIM, NAM-EUN
Owner PUKYONG NAT UNIV IND ACADEMIC COOPERATION FOUND