Compositions and methods for treating or preventing gut permeability-related disorders
a gut permeability and composition technology, applied in the field of digestive tract (gi), can solve problems such as gut barrier damag
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example 1 -
Example 1-Methods for Making GeB-01, GeB-02, GeB-03 and GeB-04
[0101]Polymers according to Table 1 were prepared as set forth in Example 1 of US 2016 / 0222134, except that for GelB-03 and GelB-04, the crosslinking time was increased as indicated in the Table 1.
TABLE 1Aver-Aver-X-link TimeageageName(time@120° C.)MURMURG′ [Pa]G′[Pa]Gel B-01Not X-linkedGel B-024 hours78, 76, 77771966, 1885,18271688Gel B-036 hours36, 36, 36365358, 5064,52935227Gel B-048 hours24, 25, 21236880, 77577319
[0102]Gel B-01, Gel B-02, Gel B-03 and Gel B-04 were prepared as follows.
[0103]For the mixing step, a homogeneous mixture of citric acid (0.2% w / w CMCNa), 7H4MF (6% w / w DI Water) carboxymethyl cellulose and DI water was obtained through planetary mixer technology. Three (3) hours of mixing were enough to prevent any lumps in the mixture. For the drying step, a thin layer of CA / CMC / Water mixture was rolled out on a silicone sheet. The homogeneity of the layer is important to promote homogeneous drying and to p...
example 2
and Methods for Characterizing Hydrogels of the Invention Using Carboxymethylcellulose (CMC) as an Example
[0122]Preparation of Simulated Gastric Fluid / Water (1:8)
[0123]Reagents used for preparation of SGF / water (1:8) solution are purified water, sodium chloride, 1M hydrochloric acid and pepsin.
[0124]1. To a 1 L graduated cylinder pour about 880 mL of water.
[0125]2. Place the cylinder on a magnetic stirrer, add a magnetic bar and start stirring.
[0126]3. Begin monitoring the pH of the water with a pH meter.
[0127]4. Add a sufficient amount of 1M hydrochloric acid to bring the pH to 2.1±0.1.
[0128]5. Add 0.2 g NaCl and 0.32 g pepsin. Leave the solution to stir until complete dissolution.
[0129]6. Remove the magnetic bar and the electrode from the cylinder.
[0130]7. Add the amount of water required to bring the volume to 900 mL.
[0131]Determination of Viscosity of Carboxymethylcellulose Solutions
[0132]Equipment and Materials:
[0133]Constant temperature water bath.
[0134]Glass Bottle, 500 ml wi...
example 3
udies
[0252]C57BL6 / J mice were purchased from Charles River Laboratories. All mice used were between 8 to 12 weeks of age at the time of the experiment. Mice were maintained at IFOM-IEO Campus animal facility under specific pathogen-free conditions. All experiments were performed in accordance with the guidelines established in the Principles of Laboratory Animal Care (directive 86 / 609 / EEC).
[0253]C57BL6 / J female and male mice at 8 weeks of age were fed with chow diet supplemented with different concentrations of Gel B-02 (2%-4%-6%-8%) and the respective control chow diet (4RF21 repelletted, Mucedola srl) for 4 weeks. The description of Gel B-02 is found in Table 1 of Example 1.
[0254]After 4 weeks of feeding mice were morning fasted for 6 hours and blood samples were collected from the tail vein through a small cut with a sharp scalpel. A drop of blood was directly used to measure glucose levels using a hand-held whole-blood glucose monitor from Roche (Accu-Chek Aviva, Roche), and oth...
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