A high-frequency and high-voltage pulse generator for sterilization relates to a pulse generator. A control circuit and a pulse generating circuit are provided; the control circuit is provided with a voltage comparator, a triode, a first NAND gate, a second NAND gate, a coupler, and a relay; the pulse generating circuit is provided with a relay normally open switch, MOSFET switching tube, first transformer, second transformer, diode, capacitor, spark arrestor. Based on the traditional high-frequency pulse device, a timer based on NE555 is designed, and DSP is used to digitally process the sterilization signal, so that the interference of the sterilization circuit is greatly reduced. Capacitor charging and discharging is used to obtain high-voltage pulses, so that the output pulse can reach 12-100kV/cm, and the width is controlled at the microsecond level, and the pulse front is controlled at the nanosecond level, which can effectively sterilize and will not change the food composition. ; It can be turned on and off in an instant and has a large flow capacity and reliable operation.