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Method for producing soybean trypsin inhibitor concentration

A technology of trypsin inhibition, manufacturing method, applied in the field of useful concentrates, which can solve the problems of lack of effective utilization of various components, and impossibility of industrialization

Inactive Publication Date: 2007-11-14
FUJI OIL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] On the other hand, although examples of the separation method of soybean trypsin inhibitor and the separation method of soybean β-amylase in the prio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0031] Add 10 times the amount of warm water to low-denatured defatted soybeans to maintain pH = 7.0 while stirring for 1 hour for extraction. Okara which is an insoluble component was removed with a decanter to obtain a defatted soybean emulsion. Concentrated hydrochloric acid was added to the defatted soybean emulsion to make the pH = 4.5, and the precipitated soybean protein was separated to obtain soybean whey.

Embodiment 1

[0033]The soybean whey (solid content: 3% by weight, pH = 4.5) obtained in Production Example 1 above was subjected to a vacuum degree of 40 Torr, a heating temperature of 60° C., and an evaporation temperature of 35° C. using a thin-film flash evaporator (manufactured by Tokyo Rike Instruments Co., Ltd.). Concentrate under reduced pressure under certain conditions, and extract when the solid content concentration is 7%, 12%, 16%, and 27%. Sodium sulfate was added to the obtained concentrated whey of various concentrations in an amount of 0.5%, 3%, 6%, 9%, and 14% of the concentrated whey, further adjusted to pH = 4 with phosphoric acid, and refrigerated for one night.

[0034] Each sample was centrifuged at 3,000 rpm for 5 minutes, and the supernatant was subjected to SDS electrophoresis to quantify the concentration of the 20 kDa band corresponding to Kunitz-type trypsin inhibitor (hereinafter referred to as KSTI), and compare it with the whey of the raw material.

Embodiment 2

[0035] Example 2 (the example where the whey concentration is 28%)

[0036] In the same manner as in Example 1, the soybean whey was concentrated under reduced pressure in a thin-film flash evaporator to a solid content concentration of 28%. Sodium sulfate is added to the concentrated whey in an amount of 3% or 6% of the concentrated whey, further adjusted to pH = 4 with phosphoric acid, and stored overnight in refrigeration.

[0037] Each sample was centrifuged at 3000 rpm for 5 minutes, and the supernatant was subjected to SDS electrophoresis, and the concentration of the 20 kDa band corresponding to KSTI was quantified, and compared with the whey of the raw material.

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PUM

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Abstract

To provide a method of simply and inexpensively manufacturing a useful concentrate of a trypsin inhibitor and the like contained in soybeans and having a physiological activity.In the manufacturing method of the soybean trypsin inhibitor cocentrate, a soybean whey is concentrated to 10 to 30 wt.% in solid content concentration, 3 wt.% or more of a salt is added to the concentrated soybean whey to recover agglomerated precipitate precipitated under an acid condition.

Description

technical field [0001] The present invention relates to a method for easily and inexpensively producing useful concentrates such as trypsin inhibitors and the like contained in soybeans. Background technique [0002] Trypsin inhibitor is one of the physiologically active substances contained in soybeans. It is known that there are Kunitz type and BBI type. The molecular weight of Kunitz type is about 20000, and the isoelectric point is 4.5. It is 4.2, and it is reported that there are cancerous thoracic-ascites retention inhibitors (Japanese Patent Publication No. 6-23113 ), inflammatory edema hyperactivity inhibitors (Japanese Patent Application Publication No. 7-10772 ), and enzyme-containing inhibitors that exert physiological effects. Disposable absorbent products of skin care compositions (JP-A-2002-505916) and the like. [0003] As separation methods for soybean trypsin inhibitors, various purification methods have been proposed by combining laboratory-level isoelectr...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/81C12N9/50C12N9/14C12N9/99A23C21/00A23J3/16A23L2/00A61K36/48A61P17/00A61P29/00A61P43/00
Inventor 吉田隆治福田洋一
Owner FUJI OIL CO LTD