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Method for preparing medical collagen protein powder

A protein powder and collagen technology, which is applied in the field of biomedical material preparation, can solve the problems of complex extraction process, high production cost, limited resources, etc., and achieves the effects of low operating cost, short production cycle and maintaining biological activity.

Inactive Publication Date: 2007-11-14
成都维德医疗器械有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. Only cowhide, bovine tendon and rat tail tendon are considered as raw materials, and their resources are limited, and there is a risk of carrying mad cow disease virus;
[0009] 2. Enzymatic extraction is mostly used only, and the extraction process is complicated and the cycle is long;
[0010] 3. The yield is low, generally below 16% (dry weight basis);
[0011] 4. High production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Pretreatment: Take 100 parts by weight of purified pigskin, put it into a drum, add 300 parts by weight of deionized water with a temperature of 32 ° C, 4 parts by weight of sodium chloride, and rotate for 60 minutes; control the waste liquid, add the temperature 300 parts by weight of deionized water at 32°C, add 1 part by weight flatly, and rotate for 60 minutes; control dry waste liquid, add 300 parts by weight of deionized water, and rotate for 30 minutes; control dry waste liquid, add 300 parts by weight of deionized water, and rotate 30min;

[0063] (2) Segmentation: Take out the pretreated skin base from the drum, dehydrate it with a centrifuge, and then divide it into small pieces of 2-6×2-6cm;

[0064] (3) Freeze-drying: put the small pieces of skin into the freeze dryer, set the cooling parameter to drop 2°C / min until the temperature of the cold trap drops to -34°C, evacuate to 45-150mbar for 24h, and then slowly raise the temperature for 8h , so that the...

Embodiment 2

[0076] (1) Pretreatment: Take 100 parts by weight of purified pigskin, put it into a drum, add 450 parts by weight of deionized water with a temperature of 36 ° C, 7 parts by weight of sodium chloride, and rotate for 120 minutes; control the waste liquid, and then add 450 parts by weight of deionized water at a temperature of 36°C, add 3 parts by weight flatly, and rotate for 120 minutes; control dry waste liquid, add 450 parts by weight of deionized water, and rotate for 35 minutes; control dry waste liquid, add 450 parts by weight of deionized water, Rotate for 35min;

[0077] (2) Segmentation: Take out the pretreated skin base from the drum, dehydrate it with a centrifuge, and then divide it into small pieces of 2-6×2-6cm;

[0078] (3) Freeze-drying: Put the small pieces of skin into a freeze-dryer, set the cooling parameter to drop 2°C / min until the temperature of the cold trap drops to -34°C, evacuate to 45-150mbar for 24h, and then slowly raise the temperature for 12h ,...

Embodiment 3

[0090](1) Pretreatment: Take 100 parts by weight of purified pigskin, put it into a drum, add 600 parts by weight of deionized water at a temperature of 40°C, 10 parts by weight of sodium chloride, and rotate for 150 minutes; control the waste liquid, and then add 600 parts by weight of deionized water at a temperature of 40°C, add 5 parts by weight flatly, and rotate for 150 minutes; control dry waste liquid, add 600 parts by weight of deionized water, and rotate for 40 minutes; control dry waste liquid, add 600 parts by weight of deionized water, Rotate for 40min;

[0091] (2) Segmentation: Take out the pretreated skin base from the drum, dehydrate it with a centrifuge, and then divide it into small pieces of 2-6×2-6cm;

[0092] (3) Freeze-drying: Put the small pieces of skin into a freeze-dryer, set the cooling parameter to drop 2°C / min until the temperature of the cold trap drops to -34°C, vacuumize to 45-150mbar for 24h, and then slowly raise the temperature for 8 -15h, ...

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Abstract

This invention discloses a method for preparing medical collagen powder characterizing in applying purified pigskins as the raw material to be pre-processed, divided, frozen and dried, crushed, dipped in acid, then digested for 4-12h by 0.5-4.0wt% compound enzyme skin collagen process agent E to get a rough product to be refined, classified and dried to get the pure medical collagen protein powder.

Description

1. Technical field [0001] The invention relates to a preparation method of medical collagen powder, which belongs to the field of preparation of biomedical materials. 2. Background technology [0002] Collagen is an extracellular matrix, which belongs to structural protein, accounts for about 1 / 3 of the total protein in mammals, and is the main component of connective tissues or organs such as skin, ligaments, cartilage, and tendons. Currently confirmed collagen has 26 types, of which type I is the most abundant (Pace James M, Corrado Marcella, Missero Caterina. Identification, characterization and expression analysis of a new fibrillar collagen gen. Matrix Biology, 2003, 1(22): 3-14 ). The special quaternary structure of collagen determines its unique physical and chemical properties and excellent biocompatibility, degradability, low immunogenicity, cell adaptability, cell proliferation, and promotion of blood coagulation (but Weihua , Wang Kunyu, Zeng Rui, et al., Medica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02A61L15/32A61L31/04A61L33/12A61K47/42
Inventor 但卫华曾睿林海但年华米贞健
Owner 成都维德医疗器械有限责任公司
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