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Method for measuring density of organic phosphorus insecticide

A determination method and organophosphorus technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., can solve the problems of high cost, unfavorable field detection, etc. Conductive effect

Inactive Publication Date: 2007-11-14
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chromatographic technology has been used to detect the specificity of organophosphorus pesticides, but this detection method requires professional detection personnel to operate, and the sample needs to be pretreated before detection, which is costly and not conducive to Field detection

Method used

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  • Method for measuring density of organic phosphorus insecticide
  • Method for measuring density of organic phosphorus insecticide
  • Method for measuring density of organic phosphorus insecticide

Examples

Experimental program
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Embodiment 1

[0027] Embodiment 1: as shown in the figure, peristaltic pump 1 is used for inputting pesticide solution, substrate, load and multiple enzyme buffer solution in reaction tank, and working electrode 3 is the electrode that is combined with enzyme, and auxiliary electrode 2 and The reference electrode 4 is connected to the electrochemical analysis instrument 7 together, and the three electrodes are placed in the reaction cell 5; the electrochemical analysis instrument 7 is connected to the computer 8, and the measurement signal output by the electrochemical analysis instrument is analyzed and processed by the computer. In the present embodiment, the insecticide phosphide is used as the detection object, the enzyme used is acetylcholinesterase, the substrate is acetylthiocholine chloride, and the carrier liquid is a 0.1M pH7.4 PBS solution containing 0.1M KCl. The enzyme buffer is pralidoxime solution, and the BAS100B electrochemical analysis instrument is used.

[0028] In order...

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Abstract

The present invention discloses the measurement method of organophosphorus pesticide concentration. The method includes mainly preparing biosensor, measurement to obtain oxidation current I0 of choline and suppression factor I% of organophosphorus on enzyme activity, and detecting the concentration of the same kind of organophosphorus pesticide based on the linear relation between the suppression factor I% and the organophosphorus pesticide concentration. The present invention is simple, feasible and low in cost, and has fast detection speed and high efficiency.

Description

1. Technical field [0001] The invention relates to a method for measuring pesticides, in particular to a method for measuring organophosphorus pesticides. 2. Background technology [0002] Organophosphorus compounds are widely used in agriculture and other fields, such as vegetation control, lawn insecticide and so on. These compounds are very toxic and can irreversibly inhibit the activity of enzymes, thereby affecting the function of the human central nervous system, often causing paralysis of the respiratory system, and even death in severe cases. Therefore, the widespread use of organophosphorus compounds directly threatens human health. In order to prevent organophosphorus compounds from affecting human health during use, it is necessary to detect them. Therefore, it is extremely important to develop rapid, sensitive, specific and stable organophosphate detection methods. At present, chromatographic technology has been used to detect the specificity of organophosphoru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327C12Q1/46G01N27/49
Inventor 鞠熀先维卫克·巴布·坎地马拉陈进
Owner NANJING UNIV
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