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Process for preparing fuel ethanol by using straw fiber materials

A fuel ethanol and fiber-like technology, which is used in fuels, liquid carbon-containing fuels, petroleum industry, etc., can solve the problems of inadequate pretreatment methods for raw materials, high ethanol costs, and affecting the yield of ethanol fuels.

Inactive Publication Date: 2009-06-10
HENAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above process can use crop straw to produce fuel ethanol, but due to the inadequate pretreatment method of raw materials, the output rate of ethanol fuel is affected, so that the cost of ethanol production is too high

Method used

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Experimental program
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Embodiment Construction

[0018] A process for producing fuel ethanol from straw-like fiber raw materials, comprising the following steps: (1) raw material selection: crop straws, after natural drying, for later use; (2) crushing: the crop straws are pulverized by a hammer mill and sieved into 20-40 (3) pretreatment; (4) dual-enzyme saccharification: use cellulase and xylanase to saccharify the straw at 45°C, pH 4.5, 150rpm, and a liquid-solid ratio of 15. Obtain reducing sugar; (5) Filtration: the saccharification solution of crop straws is filtered to remove solid insolubles and then become a clear saccharification solution; (6) Sterilization: the saccharification solution adjusted by adding nutrients needs to pass through 121 ° C for 30 minutes Sterilization; (7) Expansion cultivation and application of Pachysolen tannophilus P-01: expand cultivation of P-01 with basal medium, and then insert 10% inoculum into fermentation medium for Fermentation; basic medium is A (g / 100ml): MgSO 4 ·7H 2 O 0.05, ...

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Abstract

This invention discloses a method for producing fuel ethanol from a straw fibre raw material, comprising: (1) raw material selection; (2) disintegration; (3) pre-processing; (4) double-enzyme saccharifying; (5) sterilization; (6) enlargement culture and application of Pachysolen tannophilus P-01; (7) fermentation; (8) distillation; characterizing: the pre-processing employs white-rot fungi solid culture to decompose lignin. The procedures comprise: (1) bacterium: Coridus versicolor; (2) crude laccase production: (1) culture media producing condition,(2) fermentation: inoculate 6 parti-color Coriolus versicolor seed stopper per 1000ml, 28-30Deg C, 150rpm shake culture for 7d, remove the hypha by filtration so as to obtain the crude laccase; (3) crude peroxidase production: i. culture media, ii. fermentation: inoculate 6 rings of Phanerochete chrysosporium spores per 100ml, 37-39Deg C, static culture for 9d, remove hypha by filtration so as to obtain crude peroxidase; (4) conditions of enzyme liquid decomposing straw: add 1ml crude peroxidase and crude laccase respectively to the straw powder and mix homogenously, and then decompose the straw at 45Deg C, pH 4.5 for 72h. This invention can improve the ethanol yield and decease cost.

Description

technical field [0001] The invention relates to a production method of liquid fuel, in particular to a process for producing fuel ethanol by using crop straw fiber raw materials. Background technique [0002] In the context of the serious crisis and serious environmental pollution caused by traditional fossil energy such as coal and oil, fuel ethanol has attracted the attention of the government and society for its advantages such as renewable, non-environmental pollution, and technical clearance in motor vehicles. However, traditional fuel ethanol production mainly uses starchy raw materials such as wheat, corn, and dried potatoes, or sugary raw materials such as sugarcane molasses and beet molasses, which require a large amount of crops and high production costs. my country is a large agricultural country with abundant plant fiber resources. The annual output of crop straw alone reaches 700 million tons. The current utilization is mainly for cooking in rural areas, some ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10L1/02
Inventor 张百良宋安东
Owner HENAN AGRICULTURAL UNIVERSITY