Process for extracting leukasmus rhabdovirus genome DNA of prawn
A white spot baculovirus and extraction method technology, applied to the field of shrimp white spot baculovirus genome DNA extraction, can solve the problems of troublesome operation and low yield of virus genome DNA extraction, and achieve convenient acquisition, easy grinding, and rapid homogenization while full effect
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[0019] Take live shrimp or fresh dead shrimp suffering from white spot disease as samples for extracting WSSV-DNA, wash them with tap water, and then rinse them with sterile water; put the samples in a heating vessel, add sterile water to immerse 2.5cm, heat to boiling, and keep 10 minutes; take the whole shrimp from the juvenile shrimp, and take the abdominal muscle from the adult shrimp, weigh 5 grams of the sample, add weight Compare 10 times (W / V=mg / ml) TN buffer (50mmol / L Tris HCl, pH7.6, 0.4mol / L NaCl) was ground to make a homogenate; Centrifuge for 10 minutes, take 1ml of the supernatant, add proteinase K to a final concentration of 100ug / ml, add KCl to a final concentration of 50mmol / L, add pH 8.3-8.5 Tris·HCl to a final concentration of 10mmol / L, add gelatin to The final concentration is 0.1mg / ml, add NP-40 to a final concentration of 0.45%, add Toween-20 to a final concentration of 0.45%, add water to make up to 2ml. Mix the above mixture, boil in boiling water f...
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