The invention discloses a method for extracting genome DNA of prawn white spot baculovirus. It takes the surviving sick shrimp or fresh dead shrimp suffering from white spot baculovirus disease as samples, washes them with sterile water first, then puts the samples in a heating vessel, adds sterile water and immerses them to 2-3cm, and heats them. After boiling, keep for 8-10 minutes, add TN buffer and grind to make a homogenate, and process through medium-speed centrifugation and other steps to obtain WSSV genomic DNA. Because the present invention adopts the method of heating and boiling to pretreat the sample, the sample is easily ground, and the homogenate is rapid and sufficient; also because after the sample is heated, the animal protein, including the viral protein, coagulates and denatures, and the nucleocapsid of the virus particle is easy to separate from the tissue, The DNA yield is greatly improved; at the same time, for the early detection of diseased shrimp such as seedlings, whole shrimp can also be used, which facilitates the acquisition of samples. The invention provides a fast, convenient and effective way for extracting WSSV genome DNA.