Biological chip of identifying colon canceration degree using microchanging of RNA
A microRNA and biochip technology, applied in the biological field, can solve the problems of lack of specificity and no obvious symptoms in the early manifestations of colon cancer, and achieve the effect of simple and clear results, accurate results, and easy preparation
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[0013] 1. MicroRNA biochip experiment
[0014] In order to screen stable and credible microRNA probes, we have done microRNA biochip experiments, which contain more than 400 probes of mature human microRNAs. SmartArray TM Dots were made on a 75×25mm, chemically modified slide. The samples spotted on the chip also included U6 and tRNA as internal standard, external standard with a length of 30 bases artificially prepared, Hex as positive control for spotting, and 50% DMSO as negative control for hybridization. The entire dot array is divided into 4 sub-arrays, each sub-array has 23 rows and 21 columns, the dot pitch is 185 μm, and the diameter of the dot is about 130 μm, and each probe is repeated three times.
[0015] The specific experimental procedures are as follows: (1) Extraction of total RNA from tissue blocks: Extract total RNA from colon cancer tissues and normal tissues by Trizol method, concentrate RNA by isopropanol precipitation, quantify with spectrophotometer, ...
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