Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature

Abstract

A method for culturing the seeds of photosensitive and thermo-sensitive male sterility line of low-temp sterile or short-day low-temp sterile rice mainly includes the following steps: 1) choosing male parent and female parent and sowing, wherein the photosensitive and thermo-sensitive sterility line of low-temp sterile or short-day low-temp sterile is taken as the female parent; 2) during young panicle fertility transform sensitive stage of female parent, irrigating with cold water to lower the temperature of panicle when the ambient temperature is higher than the critical temperature of fertility transform of female parent, in order to keep the young panicle at the temperature which is lower than critical temperature of fertility transform and maintain completely male sterility; 3) hybridizing male parent and female parent; 4) growing and harvesting. The invention can resolve the problems of sterility and instability generated during culturing the seeds of photosensitive and thermo-sensitive sterility line of low-temp sterile or short-day low-temp sterile type rice, and can artificially control sterility of the rice seeds. The invention can also improve the breeding efficiency of the good combining ability photosensitive and thermo-sensitive sterility line and the various types of strong-good hybridization combination.

Description

technical field [0001] The present invention relates to the field of breeding. Specifically, the present invention relates to a seed production method of a low-temperature sterile or short-day low-temperature sterile type rice photothermosensitive genetic male sterile line. Background technique [0002] Rice is a self-pollinating plant, but hybrid rice has long been found to have heterosis including many desirable traits. Therefore, male sterile lines are widely valued in the field of breeding because they can avoid manual emasculation, save manpower, reduce seed costs, and ensure seed purity. Photothermosensitive sterility is a common phenomenon in nature. The photothermosensitive genic male sterile line has obvious fertility conversion characteristics, and its fertility expression is regulated by light and temperature factors. The fertility sensitive period is under certain temperature and light conditions, and the male development is sterile; the fertility sensitive per...

AI Technical Summary

Problems solved by technology

The low-temperature sterile or short-day low-temperature sterile type photothermo-sensitive genic male sterile line is only suitable for seed production in areas with low temperature (such as areas with high altitude) during the fertility-sensitive period of rice panicle differentiation and development, and its application is greatly affected. limit

Therefore, since the low-temperature sterile or short-day low-temperature sterile rice photothermosensitivity male sterile line is more risky in seed production than the high-temperature sterile or long-day high-temperature sterile photothermosensitivity male sterile line, it is rarely used at present. Their seed production safety issues need to be addressed