Method for preparing water-insoluble beta-1,3/1,6-dextran

Abstract

The invention discloses a method for preparing water-insoluble beta-1, 3 / 1, 6-qlucan. The method has the steps as follow: 1)adding lysozyme into yeast cell wall suspending liquid for enzymolysis, centrifugation and collection at a temperature between 35 and 40 DEG C; 2) putting the product achieved from step 1) into NaOH solution and stirring for centrifugation and collection at a temperature between 35 and 4 DEG C; 3) depositing the product achieved from step 2) into acetic acid solution having concentration expressed in percentage by volume of 2-8 percent and stirring for centrifugation andcollection; 4) stirring and washing the deposition after acid treatment and centrifugation of the step 3)by absolute ethyl alcohol and acetone and ether extract sequentially, and laying aside the treated ether extract for 12 to 24 hours, and drying in the centrifugation and collection way to achieve water-insoluble beta-1, 3 / 1, 6-qlucan. The invention is simple in control, and the beta-1,3-qlucanand the 1,6-qlucan prepared by the method have high purity and yield, and has the protein content and the fat content less than 1 percent.

Description

technical field

[0001] The invention relates to a preparation method of water-insoluble β-1,3 / 1,6-glucan. Background technique

[0002] Yeast is a single-celled fungus and is the most widely used and economically valuable microorganism so far. The international FDA recognizes brewer's yeast as a safe microorganism and has been widely used in the production of beverages and foods. At present, my country consumes about 2,000 tons of yeast annually and produces about 3 tons of waste yeast sludge. Since the yeast cell wall is the main by-product of the beer manufacturing industry, and the main treatment method for it today is to sell it cheaply as roughage or discharge it directly, it causes a lot of waste of resources and pollution to the environment.

[0003] Several research results have shown that pure yeast cell walls have obvious immune-boosting effects on animals, and also exhibit growth-promoting effects. Further evidence shows that the main mechanism is that the β-1,...

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