A method for separating and purifying lentinan

A technology for the separation and purification of lentinan, which is applied in food preparation, food science, application, etc., can solve the problems of high viscosity of the solution, clogging that cannot reach adsorption separation, easy clogging, etc., and achieves a simple method, quality assurance, and low cost. Effect

Inactive Publication Date: 2008-04-16
上海慈瑞医药科技股份有限公司
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Problems solved by technology

The difficulty encountered in the original method is: because the lentinan is difficult to dissolve, the viscosity of the solution is large, and the traditional chromatography column is formed by connecting the column interface 1 and the cylinder 2, such as figure 1 As shown, at >0.25% dissolved content, the throughput can only reach 500mg / cm 2 If it is too high, it is easy to block, because in the crude product solution, if the dissolved content is large, the viscous solution of lentinan gradually accumulates on the critical surface of DEAE-cellulose, which cannot circulate, and the formation of blockage cannot achieve the effect of adsorption and separation

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Embodiment

[0016] Such as figure 2 Shown is a schematic diagram of the structure of the chromatographic column of the present invention. The special-shaped chromatographic column is composed of a column interface 1 connected to an upper and a lower funnel 2 and a cylinder 2, and the maximum diameter of the funnel is three times the diameter of the cylinder. A method for separating and purifying lentinan is:

[0017] Step 1. The crude lentinan is pretreated

[0018] Heat lentinan and raw sugar to 50°C-99°C according to 2% dissolved content, dissolve in water and cool, tie a dialysis bag at room temperature, dialyze with tap water for ≥60H, and concentrate the dialysate to 1 / 6-1 / 2 of the original volume Within the range of 9, under the condition of stirring at room temperature, add medicinal ethanol for precipitation at a rate of 1:1.1. After the precipitate is separated, wash with medicinal ethanol twice the volume of the precipitate, and then separate the precipitate again. vacuum dry...

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Abstract

The present invention relates to a method for separating and purifying lentinan, which is characterized in that after transforming the column type of the traditional chromatographic column to heterotype column, the lentinan coarse product is pretreated so as to ensure it to adopt the absorption of the heterotype column, the separating and purifying of the lentinan is changed to that of the lentinan dynamicly and high efficiently, thus to obtain single lentinan after a series of treatment of washing, elution, dialysis, deposition and freeze out etc. The advantage of the present invention is that it is suitable for producing large quantities and industrialisation, and can obtain high effect, low cost product with the precondition of method is simple as well as quality is good.

Description

technical field [0001] The invention relates to a method for separating and purifying lentinan, which can be used for the treatment of malignant tumors such as digestive system, and belongs to the technical field of separation and purification of lentinan. Background technique [0002] Lentinan is a single polysaccharide extracted, separated and purified from the artificially cultivated mushroom mushroom fruiting bodies. As an anti-tumor drug, it has its special advantages: it can regulate immune function, and the dosage is low, the curative effect is good, and the toxicity and side effects are small. Mainly for the treatment of malignant tumors of the digestive system. It is often used in conjunction with chemotherapy drugs to avoid adverse reactions caused by chemotherapy. It is especially suitable for inoperable or recurrent gastric cancer patients, improving the quality of life and prolonging the life of patients. [0003] The extraction, separation and purification o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A23L1/29A61K36/06A23L33/125
Inventor 金幸朱益锋王立峰
Owner 上海慈瑞医药科技股份有限公司
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