Surface-located streptococcus pneumoniae polypeptides

A Streptococcus pneumoniae, a variety of polypeptide technology, applied in the direction of antibacterial drugs, bacterial antigen components, resistance to vector-borne diseases, etc., can solve problems such as non-identification of allelic variants

Inactive Publication Date: 2008-04-16
ACE BIOSCIENCES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibodies raised against a single protein may not recognize allelic variants

Method used

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  • Surface-located streptococcus pneumoniae polypeptides
  • Surface-located streptococcus pneumoniae polypeptides
  • Surface-located streptococcus pneumoniae polypeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0537] Strategy:

[0538] The planned experimental steps are as follows: surface proteins were isolated by high pH elution or by mutanolysin digestion. Isolated surface proteins were identified by three complementary mass spectrometry-based strategies: 1) 2-D SDS PAGE, 2) 1D SDS PAGE, and 3) in-solution digest. All three strategies include protein identification by mass spectrometry analysis. The identified surface proteins were cloned into E. coli expression vectors. The expressed recombinant protein was purified and used to immunize mice to confirm the immunogenicity of the antigen. The immunized mice are used in challenge studies in which mice are challenged with S. pneumoniae and monitored for protection against disease and / or death.

[0539] mouse:

[0540] Six-week-old BALB / c mice were housed under specific pathogen-free conditions and given sterile food and water ad libitum.

[0541] bacteria:

[0542] E. coli Top10 (Invitrogen) was used as a host for routine plas...

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Abstract

The present invention relates to cell-surface-located polypeptides of Streptococcus pneumoniae and their use in immunisation against Streptococcal infection, in diagnosis of Streptococcus and in identification of compounds with anti-Streptococcus activity. In a further aspect, the invention relates to antibodies capable of recognising cell surface-located polypeptides of Streptococcus pneumoniae and uses thereof.

Description

[0001] All patent and non-patent references cited in this application are hereby incorporated by reference in their entirety. This patent application claims the benefit of priority to US Provisional Application Serial No. 60 / 653,932, filed February 18, 2005, which is incorporated herein by reference in its entirety. field of invention [0002] The present invention relates to a cell surface localized polypeptide of Streptococcus pneumoniae and its use in immunization against streptococcus infection, diagnosis of streptococcus and identification of compounds with anti-streptococcus activity. Background of the invention [0003] occurrence of streptococcal infection [0004] Streptococcus pneumoniae was first identified by Sternberg and Pasteur and was originally described as pneumococcus (Austrian R. The pneumococcus at the millennium: not down, not out. J Infect Dis 1999; 179(Suppl 2): ​​S338-41). Streptococcus pneumoniae is a Gram-positive, encapsulated coccus. About 90 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61K39/40A61P31/04
CPCY02A50/30
Inventor T·科弗德N·P·耐伯格P·斯彻罗特兹-金J·皮特森A·博埃森T·A·布罗科霍瓦J·西格C·默斯科兹克
Owner ACE BIOSCIENCES AS
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