Method for preparing nano natural silk protein powder

Abstract

The invention discloses a method for preparing nanometer natural silk fibroin albumen powder. Silk fibroin albumen is dissolved in mixed solvent of 50 to 60 wt percent of NaSCN or LiBr: CH3OH: H2O (the weight ratio is 40 to 60: 22 to 42: 10 to 30), dialyzed and concentrated by water, enzymatically hydrolyzed by Alpha chymotrypsin or neutral proteinase, and finally evaporated, dried and collected through a freeze drying method or centrifugation. The size of the nano-particles obtained in the method is 80 to 500 nm. The method has the advantages that the preparation process is simple, the mass and industrialized production can be performed, and simultaneously the obtained nanometer natural silk fibroin albumen powder particles are uniform, have appropriate sizes, and are favorable for application.

Description

technical field [0001] The invention relates to a method for preparing nano-scale silk fibroin protein powder by utilizing silk fibroin, and belongs to the technical field of functional nanometer materials. Background technique [0002] Functional nanomaterials are the most dynamic field in nanomaterials science. They can be applied to high-tech fields such as chemical engineering, biology, energy, and environment, and have far-reaching impacts and broad application prospects. For example, nanobiological materials can be applied in the fields of tissue engineering, regenerative medical materials, and biocompatible interface materials. In the application of biocompatible interface materials, it mainly realizes the assembly of proteins, DNA and polyelectrolytes on many substrates under mild conditions through layer-by-layer assembly technology, which not only realizes the structure of natural biological materials Simulate, and can obtain surface layered biomaterials, solve th...

AI Technical Summary

Problems solved by technology

Although this method can produce silk fibroin powder, the particles are too large to reach the nanoscale, because CaCl 2 This metal salt, when dissolving silk fibroin, Ca ions will be complexed and wrapped in silk fibroin, and Ca ions cannot be removed even by aqueous dialysis, and this process does not remove CaCl 2 In the case of enzymolysis reaction, except that the formation of silk fibroin-Ca ion complex affects the enzymolysis reaction and reduces the enzymolysis effect, CaCl 2 The existence of the whole enzymatic hydrolysis system will also make its Ca ion form a complex structure with protease, resulting in the loss of activity of the enzyme and making it difficult for the enzymatic hydrolysis reaction to proceed normally.

That is, first enzymatic hydrolysis and then CaCl removal 2 The technical process cannot achieve the purpose of preparing nano-scale silk fibroin powder

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