Method for inducing plant regeneratation using somatic embryo of picea koraiensis nakai

A technology of somatic embryos and cellular embryos, which is applied in the field of plant regeneration induced by somatic embryos in spruce, can solve the problems of long breeding cycle and low reproduction rate of spruce spruce, and achieve rapid economic and social benefits and abundant reproductive materials , The effect of shortening the breeding cycle

Inactive Publication Date: 2008-07-30
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for inducing plant regeneration of red spruce somat

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: the method for inducing plant regeneration of spruce somatic embryos in this embodiment is realized according to the following steps: 1. Get the pretreated zygotic embryos of spruce and inoculate them with additional 6-benzylaminopurine, activator In the modified RJW medium of ketone, naphthalene acetic acid, glutamine, hydrolyzed casein, sucrose and gumide, dark culture was carried out in an environment with a temperature of 21±1°C until embryogenic callus appeared; 2. Sexual callus was transcultured and inoculated in the modified RJW medium supplemented with abscisic acid, glutamine, hydrolyzed casein, sucrose, and colloid, and cultured in the dark at a temperature of 21±1°C to somatic embryos Mature, after the cotyledons of the somatic embryos are fully developed, open the cap of the culture bottle, select a weak wind gear in the ultra-clean workbench, and use a weak wind for 5 to 7 days to dry; 3. The mature somatic embryos after drying Tran...

specific Embodiment approach 2

[0011] Specific embodiment two: The difference between this embodiment and specific embodiment one is that in step two, a weak wind gear is selected in the ultra-clean workbench, and the drying process is carried out by blowing with weak wind for 6 days. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

A plant regeneration method inducted by a somatic embryo of Korean spruce relates to a method for inducing the plant to regenerate with the somatic embryo of Korean spruce, which solves the problems that the conventional breeding of Korean spruce is of long period and low reproduction rate. The method is that: I. callus culture with zygotic embryo; II. Culturing the somatic embryo to be mature and drying the mature somatic embryo; III. Culturing the mature somatic embryo till the germination of the somatic embryo; IV. Continuing to culture until the root and stem forms, thus acquiring the regeneration plant of Korean spruce. The plant regeneration method inducted by the somatic embryo of Korean spruce of the invention uses the immature seed of Korean spruce as an explant induction somatic embryo to acquire the regeneration plant, thus shortening the breeding period of Korean spruce, the cycle is increased by 3 to 6 times compared with cottage grafting vegetative propagation, and the reproduction rate is high.

Description

technical field [0001] The invention relates to a method for inducing plant regeneration of spruce somatic embryos. Background technique [0002] Red bark spruce (Picea koraiensis Nakai), also known as red bark, is mainly distributed in Northeast China, North Korea, and the Far East of Russia. It is widely used in the construction industry, decoration industry, and paper industry. It is an important afforestation material in Northeast my country. One of the tree species. The ecological conditions in the distribution area of ​​Picea redskin are complex, which makes it difficult to build seed orchards, and the construction and operation costs are high; the breeding cycle of Picea redskin is as long as more than 40 years, and the reproduction rate is low. Its high-quality germplasm resources have been seriously lost. In recent years, some seed orchards have been built and some excellent clones that can be matured in 15 to 20 years have been bred. application. At present, red ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李成浩刘宝光张含国刘桂丰
Owner NORTHEAST FORESTRY UNIVERSITY
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