Rapid joint inspection reagent for hemoglobin -haptoglobin complex and occult blood in stool
A technology of combining globin and hemoglobin, which is applied in the field of medical diagnostic reagents and can solve the problem that anti-hemoglobin antibodies cannot be identified and other problems.
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[0023] The present invention will be further described below in conjunction with the drawings and embodiments.
[0024] 1. Sample pad treatment: Prepare the formula as shown in the following table (calculated as a percentage), spread it evenly on the sample pad and maintain it at 37°C, and dry it with a relative humidity of less than 20%.
[0025] Name
Content
Sodium Cholate
0.1%-0.7%
S17
0.05%-1%
Sodium borate (pH9.0) buffer
0.01mmol / l-0.03mmol / l
[0026] 2. Coated gold-labeled antibody colloidal gold binding pad: 100ml of 0.01% chloroauric acid solution, heated and boiled, added 3ml of 1% trisodium citrate solution and boiled for 5 minutes to obtain colloidal gold solution. Take 100ml of the colloidal gold solution that has been cooled to room temperature, add 10ml of 0.1mol / l boric acid buffer, add 1-2mg of Hb1 antibody under stirring after the addition is complete, continue to stir for 5 minutes, and then add 2ml of 10...
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