Rapid joint inspection reagent for hemoglobin -haptoglobin complex and occult blood in stool

A technology of combining globin and hemoglobin, which is applied in the field of medical diagnostic reagents and can solve the problem that anti-hemoglobin antibodies cannot be identified and other problems.

Inactive Publication Date: 2008-08-06
王学生 +1
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to make it quicker by testing how well our red cells are binding with certain molecules called heme proteins that help them bind together when they break down into smaller parts like urine. By comparing these results from different samples we get an indication about whether there's any type of disease related to this specific protein.

Problems solved by technology

In summary, technical problem addressed in this patents relates to finding potential complications associated with abnormalities found during examination procedures like histopathology tests performed before surgery. Current techniques involve invasively obtaining samples containing blood cells collected from the body's organs, but they have limitations including lack sensitivity and non-specific reactions leading to incorrect conclusions. Immunocytochromatic colorimetric assays offer an alternative solution, making it easier to identify certain types of diseases without subjecting them to biopsy.

Method used

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  • Rapid joint inspection reagent for hemoglobin -haptoglobin complex and occult blood in stool
  • Rapid joint inspection reagent for hemoglobin -haptoglobin complex and occult blood in stool
  • Rapid joint inspection reagent for hemoglobin -haptoglobin complex and occult blood in stool

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with the drawings and embodiments.

[0024] 1. Sample pad treatment: Prepare the formula as shown in the following table (calculated as a percentage), spread it evenly on the sample pad and maintain it at 37°C, and dry it with a relative humidity of less than 20%.

[0025] Name

Content

Sodium Cholate

0.1%-0.7%

S17

0.05%-1%

Sodium borate (pH9.0) buffer

0.01mmol / l-0.03mmol / l

[0026] 2. Coated gold-labeled antibody colloidal gold binding pad: 100ml of 0.01% chloroauric acid solution, heated and boiled, added 3ml of 1% trisodium citrate solution and boiled for 5 minutes to obtain colloidal gold solution. Take 100ml of the colloidal gold solution that has been cooled to room temperature, add 10ml of 0.1mol / l boric acid buffer, add 1-2mg of Hb1 antibody under stirring after the addition is complete, continue to stir for 5 minutes, and then add 2ml of 10...

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Abstract

A reagent capable of quickly and jointly detecting hemoglobin-haptogiobin compound in stool and occult blood in stool adopts the immunological principle and colloidal gold immune chromatographic technology and mainly comprises an immune test bar and a plastic piece. The reagent has great significance in clinical early diagnosis and identification of tumours with hemorrhage of lower digestive tract symptom like rectal cancer or colon cancer, etc., with simple method, convenient operation, stable performance and correct results. Because of the characteristics of quickness, simplicity and high accuracy, the reagent which is suitable for clinical medical examination and family self examination also provides means not only for screening the diseases of this category but also for diagnosing the diseases of this category in underdeveloped areas.

Description

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Claims

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Application Information

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Owner 王学生
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